Document Detail

Context-dependent differentiation of multipotential keratin 14-expressing tracheal basal cells.
MedLine Citation:
PMID:  21131447     Owner:  NLM     Status:  MEDLINE    
Multipotential (MP) differentiation is one characteristic of a tissue-specific stem cell (TSC). Lineage tracing of tracheobronchial basal cells after naphthalene (NA) injury or in the postnatal period demonstrated that basal cells were MP progenitors for Clara-like and ciliated cells. These studies, as well as reports of spatially restricted, label-retaining basal cells, and MP differentiation by human bronchial cells support the hypothesis that a TSC maintained and repaired the tracheobronchial epithelium. However, differences in basal cell phenotype (keratin [K] 5+ versus K14+), age (postnatal versus adult), health status (normal versus injured), and injury type (acid, detergent, NA) limited comparisons among studies and thus diminished the strength of the TSC argument. The finding that K14 was up-regulated after NA injury was a caveat to our previous analysis of reparative (r)K14-expressing cells (EC). Thus, the present study lineage traced steady-state (s)K14EC and evaluated differentiation potential in the normal and repairing epithelium. We showed that sK14EC were unipotential in the normal epithelium and MP after NA, sK14EC-dervied clones were not restricted to putative TSC niches, sK14EC cells were a direct progenitor for Clara-like and ciliated cells, MP-sK14EC clones accumulated over time, and sK14EC-derived Clara-like cells were progenitors for ciliated cells.
Moumita Ghosh; Heather M Brechbuhl; Russell W Smith; Bilan Li; Douglas A Hicks; Timothy Titchner; Christine M Runkle; Susan D Reynolds
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-12-03
Journal Detail:
Title:  American journal of respiratory cell and molecular biology     Volume:  45     ISSN:  1535-4989     ISO Abbreviation:  Am. J. Respir. Cell Mol. Biol.     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-08-04     Completed Date:  2011-10-03     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  8917225     Medline TA:  Am J Respir Cell Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  403-10     Citation Subset:  IM    
Division of Cell Biology, Department of Pediatrics, National Jewish Health, Denver, Colorado, USA.
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MeSH Terms
Bronchi / cytology,  metabolism
Cell Differentiation*
Cell Lineage*
Cells, Cultured
Epithelial Cells / metabolism*
Keratin-14 / physiology*
Naphthalenes / toxicity
Stem Cells / metabolism*
Trachea / cytology*,  injuries,  metabolism*
Grant Support
Reg. No./Substance:
0/Keratin-14; 0/Naphthalenes; 2166IN72UN/naphthalene

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