Document Detail


Content and thrombin-induced release of acid hydrolases in gel-filtered platelets from patients with storage pool disease.
MedLine Citation:
PMID:  1131424     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The levels of four acid hydrolases, beta-N-acetyl glucosaminidase, beta-glucuronidase, beta-galactosidase, and acid phosphatase, and the extent of their release (release II) by thrombin was determined in platelets from nine normal subjects, nine patients with storage pool disease, and in normal platelets which had been exposed to aspirin. The levels of all four hydrolases were normal in patients with SPD. However, release of three of these hydrolases (acid phosphatase was an exception) by low concentrations of thrombin (0.015 and 0.04 U/ml) was decreased in the patients as a group, although considerable variation in the extent of release of each enzyme was noted. In contrast, aspirin failed to inhibit release II in normal platelets (except for a slight impairment in the release of beta-N-acetyl glucosaminidase), although release I (serotonin, ATP and ADP) was inhibited. All release defects could be overcome by using higher concentrations of thrombin (0.2 U/ml). The normal levels of acid hydrolases in the platelets of patients with SPD (who are deficient in the platelet dense granules) suggest that these enzymes are not normally stored in the dense granules, but rather in alpha-granules. The findings also support the conclusions of previous studies that the release reaction is impaired in SPD. This release defect appears to be different from that seen in normal platelets after exposure to aspirin.
Authors:
H Holmsen; C A Setkowsky; B Lages; H J Day; H J Weiss; M C Scrutton
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Blood     Volume:  46     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  1975 Jul 
Date Detail:
Created Date:  1975-09-03     Completed Date:  1975-09-03     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  131-42     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Acetylglucosaminidase / blood
Acid Phosphatase / blood
Adenosine Diphosphate / metabolism
Adenosine Triphosphate / metabolism
Aspirin
Blood Platelet Disorders / blood,  congenital
Blood Platelets / enzymology*,  metabolism
Chromatography, Gel
Galactosidases / blood
Glucuronidase / blood
Humans
Hydrolases / blood*
Serotonin / metabolism
Thrombin
Tritium
Chemical
Reg. No./Substance:
10028-17-8/Tritium; 50-67-9/Serotonin; 50-78-2/Aspirin; 56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate; EC 3.-/Hydrolases; EC 3.1.3.2/Acid Phosphatase; EC 3.2.1.-/Galactosidases; EC 3.2.1.31/Glucuronidase; EC 3.2.1.52/Acetylglucosaminidase; EC 3.4.21.5/Thrombin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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