Document Detail


Content determination of benzyl glucosinolate and anti-cancer activity of its hydrolysis product in Carica papaya L.
MedLine Citation:
PMID:  22305790     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
OBJECTIVE: To determine the content of benzyl glucosinolate (BG) in the pulp and the seed and investigate the anti-cancer activity of its hydrolysis product in Carica papaya L.
METHODS: Determination of BG was performed on an Hypersil BDS C(18) column at the wavelength of 214 nm with 0.1% trifluoroacetic acid (TFA) aqueous solution (A) and 0.1%TFA acetonitrile (B) as the mobile phase. In vitro activity test was adopted with cultured human lung cancer H69 cell in vitro to investigate the inhibition rate of cell proliferation of benzyl isothiocyanate (BITC) against H69 cell.
RESULTS: The pulp has more BG before the maturation of papaya and it nearly disappeared after papaya matured, while the seed contains BG at every stage. Activity test demonstrated that the a higher concentration of BITC would have better inhibition rate of cell proliferation on H69 cell, and the IC(50) was 6.5 μmol/L.
CONCLUSIONS: BG also can be produced in the pulp of papaya and it will be stored in the seed after the fruit has been matured. The hydrolysis product of BG has certain cancer-prevention anti-cancer activities for human.
Authors:
Ze-You Li; Yong Wang; Wen-Tao Shen; Peng Zhou
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Asian Pacific journal of tropical medicine     Volume:  5     ISSN:  1995-7645     ISO Abbreviation:  Asian Pac J Trop Med     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-02-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101533720     Medline TA:  Asian Pac J Trop Med     Country:  Singapore    
Other Details:
Languages:  eng     Pagination:  231-3     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Hainan Medical College. Published by Elsevier B.V. All rights reserved.
Affiliation:
Agriculture School of Hainan University, Haikou 571101, P.R. China; The School of Medicine, Hainan Medical University, Haikou 571101, P.R. China; Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, P.R. China.
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