Document Detail

Contamination of river water by Cryptosporidium parvum oocysts in western Japan.
MedLine Citation:
PMID:  11525974     Owner:  NLM     Status:  MEDLINE    
In Japan, only a few rivers have been inspected for Cryptosporidium parvum contamination, and the methods used had low sensitivity. In 1998 and 1999, we used a method with higher sensitivity to examine all large rivers used as sources of water supply in one prefecture (which we divided into four areas) in western Japan for Cryptosporidium oocysts. One sample was collected at each of 156 sites along 18 rivers, and samples were tested for Cryptosporidium oocysts by immunomagnetic separation. Samples were classified as being obtained on an island with livestock and fishing industries, a densely populated urban area, a western region including farming villages, or a still more rural northern area with agriculture and fishing. Restriction fragment length polymorphism analysis was used for identification of the C. parvum found as the bovine or human type. C. parvum was detected in at least one sample from 13 of the 18 rivers and in 47% (74 of 156) of the samples. One-third to all of the samples from each area contained C. parvum oocysts. The number of C. parvum oocysts per 20 liters of river water varied in the same pattern as the number of cattle kept in the four kinds of areas (as determined by the Mantel extension test). Oocysts isolated were of the bovine type; the C. parvum detected in rivers probably came from cattle kept in that valley. As we had expected, when tested with a more sensitive method, river water in western Japan was found to be greatly contaminated with C. parvum oocysts, as reported in other countries.
K Ono; H Tsuji; S K Rai; A Yamamoto; K Masuda; T Endo; H Hotta; T Kawamura; S Uga
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  67     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-08-29     Completed Date:  2001-12-04     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3832-6     Citation Subset:  IM    
Division of Microbiology, Hyogo Prefectural Institute of Public Health, Kobe University School of Medicine, Kobe, Japan.
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MeSH Terms
Cryptosporidium parvum / classification,  growth & development,  isolation & purification*
DNA, Protozoan / analysis
Fresh Water / parasitology*
Immunomagnetic Separation
Parasite Egg Count
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Water Pollution*
Reg. No./Substance:
0/DNA, Protozoan

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