Document Detail


Contactless conductivity detection of selected organic ions in on-chip electrophoresis.
MedLine Citation:
PMID:  15004853     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The detection of underivatized anionic sulfonates, carboxylates, amino acids, sugars, and artificial sweeteners, and of cationic dopamine, ephedrine, and metanephrine in microfabricated electrophoresis devices is demonstrated. This was achieved by high-voltage contactless conductivity measurements with external electrodes. Poly(methyl methacrylate) chips with thin covers to enable sensitive contactless detection were used for most determinations but glass microchips had to be employed for amino acids and sugars. The plastic chips were found not be stable in the alkaline media required to render those two classes of species in the ionic form amenable for separation and detection. The reproducibility of peak area measurements was about 1% or better and the detection limits ranged between 1 and 30 microM for the different compounds examined.
Authors:
Jatisai Tanyanyiwa; Eva M Abad-Villar; Peter C Hauser
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Electrophoresis     Volume:  25     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-03-08     Completed Date:  2004-11-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  903-8     Citation Subset:  IM    
Affiliation:
Department of Chemistry, The University of Basel, Spitalgasse 51, CH-4004 Basel, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Alkanesulfonates / analysis,  chemistry
Amino Acids / analysis,  chemistry
Anions / analysis*,  chemistry
Carboxylic Acids / analysis,  chemistry
Cations / analysis*,  chemistry
Electric Conductivity
Electrodes
Electrophoresis, Capillary / methods*
Organic Chemicals / chemistry*
Pharmaceutical Preparations / analysis,  chemistry
Sweetening Agents / analysis,  chemistry
Chemical
Reg. No./Substance:
0/Alkanesulfonates; 0/Amino Acids; 0/Anions; 0/Carboxylic Acids; 0/Cations; 0/Organic Chemicals; 0/Pharmaceutical Preparations; 0/Sweetening Agents

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