| Contactless conductivity detection of selected organic ions in on-chip electrophoresis. | |
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MedLine Citation:
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PMID: 15004853 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The detection of underivatized anionic sulfonates, carboxylates, amino acids, sugars, and artificial sweeteners, and of cationic dopamine, ephedrine, and metanephrine in microfabricated electrophoresis devices is demonstrated. This was achieved by high-voltage contactless conductivity measurements with external electrodes. Poly(methyl methacrylate) chips with thin covers to enable sensitive contactless detection were used for most determinations but glass microchips had to be employed for amino acids and sugars. The plastic chips were found not be stable in the alkaline media required to render those two classes of species in the ionic form amenable for separation and detection. The reproducibility of peak area measurements was about 1% or better and the detection limits ranged between 1 and 30 microM for the different compounds examined. |
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Authors:
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Jatisai Tanyanyiwa; Eva M Abad-Villar; Peter C Hauser |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Electrophoresis Volume: 25 ISSN: 0173-0835 ISO Abbreviation: Electrophoresis Publication Date: 2004 Mar |
Date Detail:
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Created Date: 2004-03-08 Completed Date: 2004-11-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: Germany |
Other Details:
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Languages: eng Pagination: 903-8 Citation Subset: IM |
Affiliation:
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Department of Chemistry, The University of Basel, Spitalgasse 51, CH-4004 Basel, Switzerland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alkanesulfonates
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analysis,
chemistry Amino Acids / analysis, chemistry Anions / analysis*, chemistry Carboxylic Acids / analysis, chemistry Cations / analysis*, chemistry Electric Conductivity Electrodes Electrophoresis, Capillary / methods* Organic Chemicals / chemistry* Pharmaceutical Preparations / analysis, chemistry Sweetening Agents / analysis, chemistry |
| Chemical | |
Reg. No./Substance:
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0/Alkanesulfonates; 0/Amino Acids; 0/Anions; 0/Carboxylic Acids; 0/Cations; 0/Organic Chemicals; 0/Pharmaceutical Preparations; 0/Sweetening Agents |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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