Document Detail

Contact stimulation of fibroblasts for tenascin production by melanoma cells.
MedLine Citation:
PMID:  17013087     Owner:  NLM     Status:  MEDLINE    
Tenascin, an extracellular matrix glycoprotein, is widely expressed in the stroma of almost all types of solid tumours including malignant melanomas. On the basis of its antiadhesive character, it has been supposed that tenascin accumulation facilitates tumour cell invasion and consequent metastasis formation. We aimed to investigate the mechanism by which melanoma cells can modulate the production of tenascin by host stromal cells. The expression of tenascin in cocultures of fibroblasts and five melanoma cell lines, as well as in fibroblast monocultures treated with melanoma conditioned media, was analysed by immunofluorescent staining and image analysis. Tenascin production could not be observed in control fibroblasts or in melanoma cell monocultures. Faint labelling for tenascin could be detected in fibroblast monocultures treated with melanoma cell conditioned media while a very intense staining for tenascin could be seen in melanoma cell-fibroblast cocultures. The tenascin staining in the cocultures was associated with the fibroblasts that were in close contact with melanoma cells. The level of tenascin production around the fibroblasts in different areas of the cocultures correlated well with the density of melanoma cells. Our results indicate that tenascin production of fibroblasts in the tumour stroma is directly modulated by melanoma cells mainly through cell-to-cell contact signalling.
Balázs Adám; László Tóth; Gabriella Pásti; Margit Balázs; Róza Adány
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Melanoma research     Volume:  16     ISSN:  0960-8931     ISO Abbreviation:  Melanoma Res.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-10-02     Completed Date:  2007-03-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9109623     Medline TA:  Melanoma Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  385-91     Citation Subset:  IM    
Department of Preventive Medicine, School of Public Health, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
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MeSH Terms
Cell Adhesion Molecules / metabolism
Cell Communication
Coculture Techniques
Culture Media, Conditioned / pharmacology
Fibroblasts / metabolism*
Gene Expression Regulation, Neoplastic
Image Processing, Computer-Assisted
Melanoma / drug therapy*,  metabolism*
Microscopy, Fluorescence
Neoplasm Invasiveness
Neoplasm Metastasis
Signal Transduction
Skin Neoplasms / drug therapy*,  metabolism*
Tenascin / biosynthesis*
Reg. No./Substance:
0/Cell Adhesion Molecules; 0/Culture Media, Conditioned; 0/Tenascin

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