Document Detail


Contact printing of arrayed microstructures.
MedLine Citation:
PMID:  20425106     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel contact printing method utilizing a sacrificial layer of polyacrylic acid (PAA) was developed to selectively modify the upper surfaces of arrayed microstructures. The method was characterized by printing polystyrene onto SU-8 microstructures to create an improved substrate for a cell-based microarray platform. Experiments measuring cell growth on SU-8 arrays modified with polystyrene and fibronectin demonstrated improved growth of NIH 3T3 (93% vs. 38%), HeLa (97% vs. 77%), and HT1080 (76% vs. 20%) cells relative to that for the previously used coating method. In addition, use of the PAA sacrificial layer permitted the printing of functionalized polystyrene, carboxylate polystyrene nanospheres, and silica nanospheres onto the arrays in a facile manner. Finally, a high concentration of extracellular matrix materials (ECM), such as collagen (5 mg/mL) and gelatin (0.1%), was contact-printed onto the array structures using as little as 5 microL of the ECM reagent and without the formation of a continuous film bridge across the microstructures. Murine embryonic stem cells cultured on arrays printed with this gelatin hydrogel remained in an undifferentiated state indicating an adequate surface gelatin layer to maintain these cells over time.
Authors:
Wei Xu; Alicia M Luikart; Christopher E Sims; Nancy L Allbritton
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-04-28
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  397     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-29     Completed Date:  2010-11-09     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  3377-85     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acrylic Resins / chemistry
Animals
Biocompatible Materials / chemistry*
Cell Adhesion
Cell Culture Techniques
Cell Line
Extracellular Matrix / chemistry*
Humans
Mice
Polystyrenes / chemistry
Surface Properties
Tissue Array Analysis / instrumentation*
Grant Support
ID/Acronym/Agency:
EB007612/EB/NIBIB NIH HHS; HG004843/HG/NHGRI NIH HHS; R01 EB007612/EB/NIBIB NIH HHS; R01 EB007612-04/EB/NIBIB NIH HHS; R01 HG004843/HG/NHGRI NIH HHS; R01 HG004843-01/HG/NHGRI NIH HHS
Chemical
Reg. No./Substance:
0/Acrylic Resins; 0/Biocompatible Materials; 0/Polystyrenes; 9003-01-4/carbopol 940
Comments/Corrections

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