Document Detail

Contact activation via ICAM-1 induces changes in airway epithelial permeability in vitro.
MedLine Citation:
PMID:  17190650     Owner:  NLM     Status:  MEDLINE    
The role of ICAM-1 in contact activation of the bronchial epithelial cells is elucidated. Direct contact between epithelial cells and leukocytes is required to change transepithelial electrical resistance (TER) of the epithelium. Migration of human neutrophils across the layers of cultured human airway epithelial cells (Calu-3) or primary cow tracheal epithelial cells was induced by an fMLP gradient. Migrating neutrophils decreased TER and increased permeability to albumin. Monoclonal antibodies to ICAM-1 reduced neutrophil migration, thus reducing the changes in TER and changes in the epithelial permeability to albumin. By confocal microscopy, ERK1/2 was found to be locally activated in the epithelial cells at the sites of migration and cross-linking of ICAM-1. Blockade of ERK1/2 by PD98059 decreased the changes in TER which were induced by ICAM-1 cross-linking. Contact activation of the bronchial epithelial cells, involving ICAM-1 via local activation of ERK1/2, is an important mechanism of alteration of the bronchial epithelial permeability.
Hyon Choi; Neal W Fleming; Vladimir B Serikov
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Immunological investigations     Volume:  36     ISSN:  0882-0139     ISO Abbreviation:  Immunol. Invest.     Publication Date:  2007  
Date Detail:
Created Date:  2006-12-27     Completed Date:  2007-03-27     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8504629     Medline TA:  Immunol Invest     Country:  United States    
Other Details:
Languages:  eng     Pagination:  59-72     Citation Subset:  IM    
Children's Hospital Oakland Research Institute, Oakland, CA 94609, USA.
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MeSH Terms
Albumins / metabolism
Antibodies / immunology
Cell Movement
Cells, Cultured
Enzyme Activation
Epithelial Cells / physiology*
Intercellular Adhesion Molecule-1 / immunology,  metabolism*
Leukocytes / physiology
Lung / physiology*
Mitogen-Activated Protein Kinase 3 / metabolism
Trachea / cytology*,  physiology*
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Albumins; 0/Antibodies; 126547-89-5/Intercellular Adhesion Molecule-1; EC Protein Kinase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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