Document Detail


Consumption of virgin olive oil influences membrane lipid composition and regulates intracellular signaling in elderly adults with type 2 diabetes mellitus.
MedLine Citation:
PMID:  17389722     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We aimed to define changes in membrane fatty acids and signaling proteins induced by virgin olive oil (VOO) consumption in elderly persons with type 2 diabetes (n = 16) compared to a control group (n = 28). The fatty acid composition was determined by gas chromatography and G-protein subunits and protein kinase C alpha (PKCalpha) by immunoblotting. VOO consumption increased the monounsaturated fatty acid content in phospholipids and cholesterol esters in both groups. In contrast, saturated fatty acids were decreased only in phospholipids. The levels of Galphao, Gbeta, and PKCalpha were significantly lower in diabetics than in controls. However, whereas VOO consumption reduced Galphas, Gbeta, and PKCalpha in both groups, reduction in Galphai was observed only in diabetics. These results indicate that long-term VOO consumption modifies the fatty acid composition of plasma membrane, which influences the association of G proteins and PKCalpha with the lipid bilayer. These combined effects probably account for the positive effects of VOO on glycemic homeostasis.
Authors:
Javier S Perona; Oliver Vögler; Jose M Sánchez-Domínguez; Emilio Montero; Pablo V Escribá; Valentina Ruiz-Gutierrez
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The journals of gerontology. Series A, Biological sciences and medical sciences     Volume:  62     ISSN:  1079-5006     ISO Abbreviation:  J. Gerontol. A Biol. Sci. Med. Sci.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-28     Completed Date:  2007-05-15     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9502837     Medline TA:  J Gerontol A Biol Sci Med Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  256-63     Citation Subset:  AIM; IM    
Affiliation:
Nutrition and Lipid Metabolism, Instituto de la Grasa (CSIC), Av Padre García Tejero, 4, 41012 Sevilla, Spain.
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MeSH Terms
Descriptor/Qualifier:
Aged
Aged, 80 and over
Cholesterol Esters / analysis
Chromatography, Gas
Diabetes Mellitus, Type 2 / metabolism*,  physiopathology
Dietary Fats, Unsaturated / administration & dosage*
Erythrocyte Membrane / chemistry
Fatty Acids / analysis
Fatty Acids, Monounsaturated / analysis
Female
GTP-Binding Protein alpha Subunits / analysis
GTP-Binding Protein beta Subunits / analysis
GTP-Binding Proteins / analysis
Humans
Intracellular Signaling Peptides and Proteins / analysis*
Lipid Bilayers / analysis
Male
Membrane Lipids / analysis*
Phospholipids / analysis
Plant Oils / administration & dosage*
Protein Kinase C-alpha / analysis
Signal Transduction / physiology
Chemical
Reg. No./Substance:
0/Cholesterol Esters; 0/Dietary Fats, Unsaturated; 0/Fatty Acids; 0/Fatty Acids, Monounsaturated; 0/GTP-Binding Protein alpha Subunits; 0/GTP-Binding Protein beta Subunits; 0/Intracellular Signaling Peptides and Proteins; 0/Lipid Bilayers; 0/Membrane Lipids; 0/Phospholipids; 0/Plant Oils; 8001-25-0/olive oil; EC 2.7.11.13/Protein Kinase C-alpha; EC 3.6.1.-/GTP-Binding Proteins

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