Document Detail

Construction of varying porous structures in acellular bovine pericardia as a tissue-engineering extracellular matrix.
MedLine Citation:
PMID:  15576164     Owner:  NLM     Status:  MEDLINE    
In the study, a cell extraction process was used to remove the cellular components from bovine pericardia. Varying pore sizes and porosities of the acellular tissues were then created using acetic acid and collagenase and subsequently fixed with genipin. Biochemical analyses found that these acellular tissues with distinct porous structures consisted primarily of insoluble collagen, elastin, and tightly bound glycosaminoglycans. The thermal stability, mechanical properties, and capability against enzymatic degradation of the bovine pericardial tissue remained unaltered after cell extraction. However, following further treatment with acetic acid and collagenase, the thermal stability and capability against enzymatic degradation of the acellular tissues declined. The porous structures of the implanted samples seem to determine whether successful microvessel-ingrowth takes place. The acetic-acid- and collagenase-treated tissues, due to their high pore size and porosity, showed a large number of microvessels infiltrating into the interstices of the implanted samples. In contrast, a low density of microvessels was observed infiltrating into the acellular tissue and penetration of microvessels into the cellular tissue was never encountered.
Hao-Ji Wei; Huang-Chien Liang; Meng-Horng Lee; Ya-Chun Huang; Yen Chang; Hsing-Wen Sung
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomaterials     Volume:  26     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  2005 May 
Date Detail:
Created Date:  2004-12-03     Completed Date:  2005-05-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  1905-13     Citation Subset:  IM    
Division of Cardiovascular Surgery, Veterans General Hospital-Taichung, and College of Medicine, National Yang-Ming University, Taipei, Taiwan, ROC.
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MeSH Terms
Cell-Free System / transplantation,  ultrastructure
Extracellular Matrix / transplantation*,  ultrastructure*
Materials Testing
Microcirculation / cytology*,  physiology*
Neovascularization, Physiologic / physiology*
Pericardium / transplantation*,  ultrastructure*
Rats, Wistar
Tensile Strength
Tissue Engineering / methods
Transition Temperature

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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