Document Detail

Construction and validation of a microprocessor controlled extracorporal circuit in rats for the optimization of isolated limb perfusion.
MedLine Citation:
PMID:  15597400     Owner:  NLM     Status:  MEDLINE    
Although a few experimental approaches to isolated limb perfusion (ILP) are described in the literature, none of these animal models mimics the clinical perfusion techniques adequately to improve the technique of ILP on the basis of valid preclinical data. Therefore, we developed an ILP setup in rats allowing online monitoring of essential perfusion parameters such as temperature (in perfusate, various tissues, and rectum), pH (perfusate), perfusion pressure, and O(2) concentration (in perfusate, tissue), by a tailor-made data acquisition system. This setup permits close supervision of vital parameters during ILP. Various interdependencies, concerning the flow rate and the pressure of perfusate as well as tissue oxygenation were registered. For the measurement of pO(2) values in the perfusate and in different regions of the perfused hind limb, a novel type of microoptode based on quenching of a fluorescent dye was devised. Stable normothermic (37 degrees C) perfusion conditions were maintained at a constant perfusion pressure in the range of 40-60 mm Hg by administration of the spasmo lytic moxaverine (0.5 mg/mL of perfusate as initial dose) at a perfusate flow rate of 0.5 mL/min for 60 min. At the end of an ILP, there were no signs of tissue damage, neither concerning laboratory data (K(+), myoglobin, creatine kinase, lactic dehydrogenase) nor histopathological criteria. The reported ILP model is not only well suited to investigate the effects of hyperthermia but also to assess the efficacy of new antineoplastic approaches, when nude rats, bearing human tumours in the hind limbs, are used.
Ulrich Gürtler; Peter Fuchs; Achim Stangelmayer; Günther Bernhardt; Armin Buschauer; Thilo Spruss
Publication Detail:
Type:  Journal Article; Validation Studies    
Journal Detail:
Title:  Archiv der Pharmazie     Volume:  337     ISSN:  0365-6233     ISO Abbreviation:  Arch. Pharm. (Weinheim)     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-12-23     Completed Date:  2005-02-14     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0330167     Medline TA:  Arch Pharm (Weinheim)     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  672-81     Citation Subset:  IM    
Institute of Pharmacy, University of Regensburg, Regensburg, Germany.
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MeSH Terms
Chemotherapy, Cancer, Regional Perfusion* / instrumentation,  methods
Hydrogen-Ion Concentration
Lower Extremity / blood supply*
Models, Animal*
Muscle, Skeletal / blood supply,  metabolism
Oxygen / metabolism
Rats, Wistar
Regional Blood Flow
Reg. No./Substance:

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