Document Detail


Construction of soil environmental DNA cosmid libraries and screening for clones that produce biologically active small molecules.
MedLine Citation:
PMID:  17546026     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Culture-independent studies on environmental samples indicate that most bacteria are not readily cultured in the laboratory. The small fraction of bacteria that have been successfully cultured from environmental samples have been a very rewarding source of novel biologically active natural products. The introduction of DNA extracted directly from environmental samples into easily cultured bacteria and the screening of these large libraries for clones that produce biologically active small molecules is one means to access natural products encoded by the genomes of previously uncultured bacteria. This protocol provides detailed procedures for cloning DNA directly from environmental samples and screening these clones for the production of antibacterially active natural products. The entire protocol, from soil sample to the identification of antibacterially active environmental DNA clones, will take approximately 2 weeks.
Authors:
Sean F Brady
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nature protocols     Volume:  2     ISSN:  1750-2799     ISO Abbreviation:  Nat Protoc     Publication Date:  2007  
Date Detail:
Created Date:  2007-06-04     Completed Date:  2008-01-02     Revised Date:  2008-03-24    
Medline Journal Info:
Nlm Unique ID:  101284307     Medline TA:  Nat Protoc     Country:  England    
Other Details:
Languages:  eng     Pagination:  1297-305     Citation Subset:  IM    
Affiliation:
Laboratory of Genetically Encoded Small Molecules, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA. sbrady@rockefeller.edu
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / isolation & purification*
Bacteria / genetics*
Biological Products / isolation & purification*
Cloning, Molecular / methods
Cosmids / genetics*
Gene Library*
Soil Microbiology*
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Biological Products

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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