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Construction of recombinant plasmid carrying the lambda DNA fragment responsible for prophage integration.
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MedLine Citation:
PMID:  353729     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The recombinant DNA molecules were constructed from plasmid RSF2124 and the EcoRI fragment of lambda DNA containing the genes responsible for prophage integration. The presence of these genes in recombinant plasmids was detected genetically. lambda int-gene was shown to be expressed in either orientation of insertion in the plasmid. We found that recombinant plasmid was able to integrate into chromosome of lambda lysogens. The integration of plasmid into host chromosome was demonstrated by contransduction of chromosome and plasmid markers using generalized transducer P1 and by specialized transduction with lambda phages.
Authors:
N Strizhov; L Tikhomirova
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nucleic acids research     Volume:  5     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1978 Jun 
Date Detail:
Created Date:  1978-09-30     Completed Date:  1978-09-30     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1767-77     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Coliphages / genetics,  metabolism*
DNA, Recombinant* / metabolism
DNA, Viral* / metabolism
Deoxyribonucleases
Endonucleases
Escherichia coli / metabolism*
Molecular Weight
Nucleic Acid Hybridization
Plasmids*
Species Specificity
Transduction, Genetic
Chemical
Reg. No./Substance:
0/DNA, Recombinant; 0/DNA, Viral; EC 3.1.-/Deoxyribonucleases; EC 3.1.-/Endonucleases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Month: 6 Year: 1978
Volume: 5 Issue: 6
First Page: 1767 Last Page: 1777
ID: 342124
PubMed Id: 353729

Construction of recombinant plasmid carrying the lambda DNA fragment responsible for prophage integration.
N Strizhov
L Tikhomirova



Article Categories:
  • Research Article


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