| Construction of rat aldolase C expression plasmid and the hybrid formation between rat aldolase C and human aldolase A or B co-expressed in Escherichia coli. | |
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MedLine Citation:
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PMID: 2188264 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rat aldolase C cDNA was inserted in an Escherichia coli expression vector to construct the rat aldolase C expression plasmid, pRAC42. This plasmid produces active rat aldolase C in the transfected E. coli host cells. The characteristics of the purified enzyme, e.g. mol. wt, electrophoretic mobilities and kinetic parameters, are indistinguishable from those of authentic rat brain aldolase C. Three different tetrameric hybrid forms, C3A, C2A2 and CA3, in addition to C4 and A4, were found to be produced in the host cell when E.coli was co-transfected with expression plasmids for rat aldolase C and for human aldolase A. Similarly, the hybrid forms, C3B, C2B2 and CB3, in addition to C4 and B4, were also produced in the cells when co-transfected with the plasmids for rat aldolase C and for human aldolase B. |
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Authors:
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Y Takasaki; K Hori |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Protein engineering Volume: 3 ISSN: 0269-2139 ISO Abbreviation: Protein Eng. Publication Date: 1990 Mar |
Date Detail:
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Created Date: 1990-06-26 Completed Date: 1990-06-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8801484 Medline TA: Protein Eng Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 273-7 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Saga Medical School, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals DNA, Bacterial / genetics Escherichia coli / genetics Fructose-Bisphosphate Aldolase / biosynthesis, genetics* Gene Expression Humans Plasmids Protein Engineering Rats Recombinant Fusion Proteins* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/Recombinant Fusion Proteins; EC 4.1.2.13/Fructose-Bisphosphate Aldolase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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