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Construction of novel cytokine by fusion of chicken IL-2 signal peptide to mature chicken IL-15 and comparison of the adjuvant effects by DNA immunization against Eimeria challenge.
MedLine Citation:
PMID:  24139475     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A novel fusion cytokine was constructed by replacing signal peptide (SP) of chicken IL-15 (ChIL-15) with SP of chicken IL-2 (ChIL-2). The fusion cytokine (NChIL-15) was cloned into the expression vector pcDNA3.1(+) to generate pcDNA-NChIL-15. An animal experiment was carried out to evaluate the adjuvant effects of NChIL-15 on DNA vaccine pcDNA-3-1E against Eimeria acervulina challenge. The mRNA profiles of ChIL-2 and ChIFN-γ in spleen were characterized by means of real-time PCR. The recombinant positive eukaryotic expression plasmid pcDNA-NChIL-15 were constructed successfully. The protective effects provided by co-immunization with 100μg pcDNA-3-1E and 50μg pcDNA-NChIL-15, measured by relative body weight gain (BWG), average lesion score in duodenum and oocyst decrease ratio, showed no significant difference with 50μg pcDNA-ChIL-15 as an adjuvant on day 6 post infection (PI). However, chickens co-immunized with pcDNA-3-1E and pcDNA-NChIL-15 exhibited significant upregulated level of ChIL-2 and ChIFN-γ transcripts in spleen. Our original data suggests the constructed novel cytokine NChIL-15 could be a potential adjuvant used to enhance the immune protective effects, although the optimized dosage need to be explored further.
Authors:
Dexing Ma; Mingyang Gao; Jie Li; Chunli Ma; Guangxing Li
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-9-25
Journal Detail:
Title:  Veterinary immunology and immunopathology     Volume:  -     ISSN:  1873-2534     ISO Abbreviation:  Vet. Immunol. Immunopathol.     Publication Date:  2013 Sep 
Date Detail:
Created Date:  2013-10-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8002006     Medline TA:  Vet Immunol Immunopathol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 Elsevier B.V. All rights reserved.
Affiliation:
College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China.
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