Document Detail

Construction of mammalian artificial chromosomes: prospects for defining an optimal centromere.
MedLine Citation:
PMID:  10070257     Owner:  NLM     Status:  MEDLINE    
Two reports have shown that mammalian artificial chromosomes (MAC) can be constructed from cloned human centromere DNA and telomere repeats, proving the principle that chromosomes can form from naked DNA molecules transfected into human cells. The MACs were mitotically stable, low copy number and bound antibodies associated with active centromeres. As a step toward second-generation MACs, yeast and bacterial cloning systems will have to be adapted to achieve large MAC constructs having a centromere, two telomeres, and genomic copies of mammalian genes. Available construction techniques are discussed along with a new P1 artificial chromosome (PAC)-derived telomere vector (pTAT) that can be joined to other PACs in vitro, avoiding a cloning step during which large repetitive arrays often rearrange. The PAC system can be used as a route to further define the optimal DNA elements required for efficient MAC formation, to investigate the expression of genes on MACs, and possibly to develop efficient MAC-delivery protocols.
D Schindelhauer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  BioEssays : news and reviews in molecular, cellular and developmental biology     Volume:  21     ISSN:  0265-9247     ISO Abbreviation:  Bioessays     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-04-07     Completed Date:  1999-04-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8510851     Medline TA:  Bioessays     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  76-83     Citation Subset:  IM    
Department of Medical Genetics, Kinderpoliklinik, Ludwig Maximilians-Universitaet, Muenchen, Germany.
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MeSH Terms
Centromere / genetics*
Chromosomes, Artificial, Yeast
Cloning, Molecular
DNA / genetics*
Genetic Engineering*
Reg. No./Substance:

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