Document Detail


Construction of a lipopolysaccharide reporter cell line and its use in identifying mutants defective in endotoxin, but not TNF-alpha, signal transduction.
MedLine Citation:
PMID:  9743364     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Gram-negative bacterial LPS is a potent activator of inflammatory responses. The binding of LPS to CD14 initiates signal transduction; however, the molecular processes immediately following this event remain unclear. We engineered an LPS-inducible fibroblast reporter cell line to facilitate the use of molecular genetic techniques to study the LPS signaling pathway. A plasmid containing the human Tac Ag cDNA under transcriptional control of the human E selectin promoter was cotransfected into Chinese hamster ovary (CHO)-K1 cells together with a CD14 expression plasmid. A cell line was obtained, 3E10, which upregulated expression of Tac following stimulation with LPS. Pools of mutagenized cells were exposed to LPS and then labeled with anti-Tac mAb. Cells that failed to up-regulate Tac expression were enriched by flow cytometry. Thirty clonal mutant cell lines were identified that continued to express CD14 and bind LPS, but failed to express Tac or translocate nuclear factor-kappaB (NF-kappaB) following LPS exposure. TNF-alpha-treated mutant cells continued to express Tac and translocate NF-kappaB. An analysis of LPS-induced NF-kappaB activity in heterokaryons derived from polyethylene glycol-fused cell lines indicated that recessive mutations in genes encoding components of the LPS signaling pathway accounted for the signaling defects. To date, two complementation groups have been identified from 11 cell lines analyzed. These data demonstrate that the TNF-alpha signaling pathway diverges from the LPS pathway early in the signal-transduction cascade despite similarities in LPS- and TNF-alpha-induced responses. Identification of the genes affected in these mutant reporter cells should identify heretofore-elusive components of the LPS signaling cascade.
Authors:
R L Delude; A Yoshimura; R R Ingalls; D T Golenbock
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  161     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1998-10-06     Completed Date:  1998-10-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3001-9     Citation Subset:  AIM; IM    
Affiliation:
Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA. rdelude@bidmc.harvard.edu
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MeSH Terms
Descriptor/Qualifier:
Acute-Phase Proteins*
Animals
Biological Transport / immunology
Blotting, Northern
CHO Cells
Carrier Proteins / physiology
Cell Culture Techniques / methods
Cell Fusion / genetics,  immunology
Cell Line
Cell Separation
Cricetinae
Endotoxins / deficiency,  genetics*
Flow Cytometry
Genes, Reporter / immunology*
Genetic Complementation Test
Humans
Lipopolysaccharides / immunology*,  metabolism,  pharmacology
Membrane Glycoproteins*
Mutagenesis / immunology*
NF-kappa B / metabolism
Phenotype
Receptors, Interleukin-2 / biosynthesis,  drug effects,  genetics
Signal Transduction / genetics*,  immunology
Transfection / immunology
Tumor Necrosis Factor-alpha / genetics*,  pharmacology
Grant Support
ID/Acronym/Agency:
R01-GM54060/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Acute-Phase Proteins; 0/Carrier Proteins; 0/Endotoxins; 0/Lipopolysaccharides; 0/Membrane Glycoproteins; 0/NF-kappa B; 0/Receptors, Interleukin-2; 0/Tumor Necrosis Factor-alpha; 0/lipopolysaccharide-binding protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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