Document Detail


Construction and isolation of a transmissible retrovirus containing the src gene of Harvey murine sarcoma virus and the thymidine kinase gene of herpes simplex virus type 1.
MedLine Citation:
PMID:  6270359     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We constructed lambda recombinants containing the Harvey murine sarcoma virus genome and the thymidine kinase (tk) gene of herpes simplex virus type 1 linked to each other. The tk gene was located in a position downstream from both the long terminal repeat and the src gene of Harvey murine sarcoma virus. The DNAs of the lambda recombinants were used to transfect NIH3T3 mouse fibroblasts in order to obtain Harvey murine sarcoma virus DNA-induced foci of transformed cells. The transformed foci were superinfected with a helper-independent retrovirus, and new individual retrovirus were isolated from the superinfected foci. The new viruses could induce focus formation on NIH3T3 cells and could convert NIH3T3(TK-) cells into TK+ cells by carrying the herpes simplex virus type 1 tk gene into the TK- cells. From virus-infected cells, we isolated nonproducer foci on NIH3T3 cells and TK+ transformants on NIH3T3(TK-) cells containing one such new viral genome coding for the dual properties. The new retroviral sequence in the nonproducer cells could be rescued into virus particles at high titers by superinfection with a helper-independent retrovirus. A hybridization analysis indicated that the recombinant virus contained both the Harvey murine sarcoma virus src sequence and the tk gene sequence in a single RNA species approximately 4.9 kilobases long. We concluded that retroviruses can be used as true vectors for genes other than genes that lead to oncogenesis.
Authors:
C M Wei; M Gibson; P G Spear; E M Scolnick
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  39     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1981 Sep 
Date Detail:
Created Date:  1981-12-22     Completed Date:  1981-12-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  935-44     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Transformation, Viral
Cloning, Molecular
DNA, Recombinant*
Genes, Viral*
Genetic Vectors
Mice
Retroviridae / genetics*,  isolation & purification
Sarcoma Viruses, Murine / genetics*
Simplexvirus / genetics*
Thymidine Kinase / genetics*
Grant Support
ID/Acronym/Agency:
CA 21776/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Recombinant; EC 2.7.1.21/Thymidine Kinase
Comments/Corrections

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