Document Detail


Construction and immunogenicity of recombinant adenovirus expressing ORF2 of PCV2 and porcine IFN gamma.
MedLine Citation:
PMID:  21945259     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The capsid structural protein encoded by the gene ORF2 of Porcine Circovirus Type 2 is critical for eliciting the broad protective immunity. We constructed and characterized the recombinant adenovirus encoding the capsid and porcine IFN gamma, designated as rAd-ORF2-IFN-γ. The construct was further confirmed by the enzyme digestion, PCR, sequencing and transfection. The humoural immunity to the capsid protein, induced by the recombinant in the mice, was tested by ELISA. Notably, this recombinant induced a much better ORF2-specific antibody response than that of rAd-ORF2 alone or the adenovirus itself. Clearly, the porcine IFN gamma could strongly enhance the immunogenicity of ORF2. The results showed that the recombinant adenovirus might be an attractive candidate vaccine for preventing the disease associated with PCV2 infection.
Authors:
Liu Genmei; Luo Manlin; Chen Ruiai; Huang Hongliang; Pei Dangshuai
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Publication Detail:
Type:  -     Date:  2011-9-20
Journal Detail:
Title:  Vaccine     Volume:  -     ISSN:  1873-2518     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-9-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406899     Medline TA:  Vaccine     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
Affiliation:
Guangdong Key Laboratory of Zoonosis, College of Veterinary Medicine, South China Agricultural University, Guangdong, Guangzhou 510642, China.
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