Document Detail


Construction of a food-grade cloning vector for Lactobacillus plantarum and its utilization in a food model.
MedLine Citation:
PMID:  22990492     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The development of Lactobacillus plantarum to be used in starter cultures in the food industry has been limited because of the lack of a food-grade cloning vector for the bacterium. In this study, the plasmid pFLP1 was constructed by joining 2 DNA fragments derived from food-approved organisms. The 5.2-kb BamHI/KpnI DNA fragment of pRV566 containing the theta-type replicon of Lactobacillus sakei was ligated to the BamHI/KpnI DNA fragment of a 2.9-kb lactococcal cadmium resistance determinant amplified from pND918. The 8.1-kb newly constructed plasmid could transform L. plantarum N014, a bacteriocin-producing bacteria originally isolated from nham, a traditional Thai fermented sausage. The resulting transformant, L. plantarum N014-FLP, and its parent strain were shown to be very similar in growth rate and bacteriocin activity. In addition, the plasmid was very stable in its host bacteria under nonselective pressure for 100 generations in MRS medium and for 5 days in a nham model. These results suggest that pFLP1 is a potential food-grade cloning vector for L. plantarum.
Authors:
Pongsak Rattanachaikunsopon; Parichat Phumkhachorn
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of general and applied microbiology     Volume:  58     ISSN:  1349-8037     ISO Abbreviation:  J. Gen. Appl. Microbiol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-09-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0165543     Medline TA:  J Gen Appl Microbiol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  317-24     Citation Subset:  IM    
Affiliation:
Department of Biological Science, Faculty of Science, Ubon Ratchathani University.
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