| Construction and expression of humanized chimeric T cell receptor specific for chronic myeloid leukemia cells. | |
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MedLine Citation:
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PMID: 22447097 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Chimeric T cell receptors (chTCRs), composed of the single-chain variable fragments (scFv) of murine antibodies and human signaling molecules, are used to redirect the specificity of autologous or allogeneic T lymphocytes. To develop novel therapeutic agents for treatment of chronic myeloid leukemia (CML), we engineered a scFv from the hybridoma cell line CMA1 which produces monoclonal antibody specific against CML. The genes encoding the heavy and light chain variable regions were amplified from CMA1 cDNA and a humanized chTCR was constructed. Expression of the novel hchTCR was verified in NIH3T3 cells transduced with retroviral vectors. The results demonstrated that hchTCR can be expressed and presented on cell surface normally. These results suggest that retroviral vectors expressing hchTCR specific for CML cells may be used to redirect human T lymphocytes. |
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Authors:
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Dong Wang; Lijun Zhang; Yajuan Li; Haixia Wang; Qing Xiao; Weixi Cao; Wenli Feng |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-3-24 |
Journal Detail:
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Title: Biotechnology letters Volume: - ISSN: 1573-6776 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-3-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Clinical Hematology, Key Laboratory of Laboratory Medical Diagnostics Designated by the Ministry of Education, Chongqing Medical University, Chongqing, 400016, People's Republic of China, 704815378@qq.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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