| Construction and expression of a dual vector for chemo-enzymatic synthesis of plant indole alkaloids in Escherichia coli. | |
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MedLine Citation:
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PMID: 20432158 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A dual vector (pQE-70-STR1-SG) containing coding regions of strictosidine synthase (STR1, EC 4.3.3.2) and strictosidine glucosidase (SG, EC 3.2.1.105) from the Indian medicinal plant Rauvolfia serpentina was constructed. Functional expression of the vector in Escherichia coli cells (M15 strain) was proven by isolation of prepurified enzyme extracts, which show both STR1 and SG activities. Incubation of the enzyme in the presence of tryptamine and secologanin delivered the indole alkaloid cathenamine, demonstrating functional co-expression of both STR1- and SG-cDNAs. Cathenamine reduction by sodium borohydride leading to tetrahydroalstonine revealed the chemo-enzymatic indole alkaloid synthesis. |
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Authors:
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Joachim Stockigt; Bodo Hammes; Martin Ruppert |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Natural product research Volume: 24 ISSN: 1478-6427 ISO Abbreviation: Nat. Prod. Res. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-30 Completed Date: 2010-08-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101167924 Medline TA: Nat Prod Res Country: England |
Other Details:
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Languages: eng Pagination: 759-66 Citation Subset: IM |
Affiliation:
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College of Pharmaceutical Sciences, Institute of Materia Medica, Zhejiang University, Hangzhou 310058, P.R. China. joesto2000@yahoo.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carbon-Nitrogen Lyases
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genetics,
metabolism* Cloning, Molecular DNA, Complementary / genetics Escherichia coli / genetics* Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant / physiology* Glucosidases / genetics, metabolism* Indole Alkaloids / chemistry, metabolism* Molecular Structure Plant Proteins / genetics, metabolism Rauwolfia / enzymology*, genetics Secologanin Tryptamine Alkaloids / chemistry |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/Indole Alkaloids; 0/Plant Proteins; 0/Secologanin Tryptamine Alkaloids; 0/cathenamine; 483-04-5/ajmalicine; EC 3.2.1.-/Glucosidases; EC 3.2.1.21./strictosidine glucosidase; EC 4.3.-/Carbon-Nitrogen Lyases; EC 4.3.3.2/strictosidine synthetase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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