| Construction of an electro-enzymatic bioreactor for the production of (R)-mandelate from benzoylformate. | |
| | |
MedLine Citation:
|
PMID: 15005146 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Coupling both the electrocatalytic recycling of NADH and the enzymatic reduction of the substrate was used to produce (R)-mandelate from benzoylformate using benzoylformate reductase (BFR). The reduction of benzoylformate by BFR in combination with FAD-mediated electrolysis (at -0.5 V vs. Ag/AgCl) was complete in about 18 h and gave 47.5 mM (R)-mandelate from 50 mM substrate, while the process involving MV2+-mediated procedure (at -0.7 V vs. Ag/AgCl) produced 40 mM (R)-mandelate after 30 h. The overpotential for the NAD+ reduction could be decreased by about 0.2 V by substituting a toxic viologen derivative, MV2+, with a natural electron carrier, FAD. MV2+, however, decreased the productivity as BFR lost about 50% of its initial activity after 6 d in its presence. |
| | |
Authors:
|
Mee-Hae Kim; Sei-Eok Yun |
Related Documents
:
|
2546826 - Influence of non-esterified fatty acids on respiratory control of reconstituted cytochr... 9083096 - Apoxin i, a novel apoptosis-inducing factor with l-amino acid oxidase activity purified... 2885296 - Immunoelectron microscopic localization of d-amino acid oxidase in rat kidney and liver. 3292726 - Basis of the stereospecific preference of porcine kidney fibroblasts for d-2-hydroxy-4-... 500186 - Demonstration of lanthionine as a natural constituent of the peptidoglycan of fusobacte... 3925796 - Endothelium-dependent vasodilation by melittin: are lipoxygenase products involved? |
Publication Detail:
|
Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biotechnology letters Volume: 26 ISSN: 0141-5492 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2004 Jan |
Date Detail:
|
Created Date: 2004-03-09 Completed Date: 2004-09-28 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 21-6 Citation Subset: IM |
Affiliation:
|
Department of Food Science and Technology and Institute of Agricultural Science and Technology, Chonbuk National University, Chonju 561-756, Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bioreactors* Electrochemistry / instrumentation, methods Electrolysis / instrumentation*, methods Electromagnetic Fields Enterococcus faecalis / enzymology* Enzyme Activation Equipment Design Equipment Failure Feasibility Studies Glyoxylates / chemistry* Mandelic Acids / chemical synthesis* NAD / chemistry*, radiation effects Oxidoreductases / chemistry*, radiation effects |
| Chemical | |
Reg. No./Substance:
|
0/Glyoxylates; 0/Mandelic Acids; 53-84-9/NAD; 611-73-4/phenylglyoxylic acid; 90-64-2/mandelic acid; EC 1.-/Oxidoreductases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Microbial degradation of poly(D-3-hydroxybutyrate) by a new thermophilic streptomyces isolate.
Next Document: Promotion of the growth of Crocus sativus cells and the production of crocin by rare earth elements.