Document Detail

Construction of an electro-enzymatic bioreactor for the production of (R)-mandelate from benzoylformate.
MedLine Citation:
PMID:  15005146     Owner:  NLM     Status:  MEDLINE    
Coupling both the electrocatalytic recycling of NADH and the enzymatic reduction of the substrate was used to produce (R)-mandelate from benzoylformate using benzoylformate reductase (BFR). The reduction of benzoylformate by BFR in combination with FAD-mediated electrolysis (at -0.5 V vs. Ag/AgCl) was complete in about 18 h and gave 47.5 mM (R)-mandelate from 50 mM substrate, while the process involving MV2+-mediated procedure (at -0.7 V vs. Ag/AgCl) produced 40 mM (R)-mandelate after 30 h. The overpotential for the NAD+ reduction could be decreased by about 0.2 V by substituting a toxic viologen derivative, MV2+, with a natural electron carrier, FAD. MV2+, however, decreased the productivity as BFR lost about 50% of its initial activity after 6 d in its presence.
Mee-Hae Kim; Sei-Eok Yun
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology letters     Volume:  26     ISSN:  0141-5492     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2004-03-09     Completed Date:  2004-09-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  21-6     Citation Subset:  IM    
Department of Food Science and Technology and Institute of Agricultural Science and Technology, Chonbuk National University, Chonju 561-756, Korea.
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MeSH Terms
Electrochemistry / instrumentation,  methods
Electrolysis / instrumentation*,  methods
Electromagnetic Fields
Enterococcus faecalis / enzymology*
Enzyme Activation
Equipment Design
Equipment Failure
Feasibility Studies
Glyoxylates / chemistry*
Mandelic Acids / chemical synthesis*
NAD / chemistry*,  radiation effects
Oxidoreductases / chemistry*,  radiation effects
Reg. No./Substance:
0/Glyoxylates; 0/Mandelic Acids; 53-84-9/NAD; 611-73-4/phenylglyoxylic acid; 90-64-2/mandelic acid; EC 1.-/Oxidoreductases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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