Document Detail


Construction and delivery of tissue-engineered human retinal pigment epithelial cell sheets, using magnetite nanoparticles and magnetic force.
MedLine Citation:
PMID:  15869427     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Choroidal neovascularization (CNV) is the most severe form of age-related macular degeneration (AMD), which causes rapid visual loss. Transplantation of cultured retinal pigment epithelium (RPE) cell sheet by tissue engineering is a possible approach to the treatment of CNV. In the present study, we investigated the possibility of using magnetite nanoparticles and magnetic force to construct and deliver RPE cell sheets in vitro. When magnetite cationic liposomes (MCLs), having a positive charge at the surface, were added to ARPE-19 human RPE cells at a concentration of 25 or 50 pg of magnetite per cell, the cells took up 40 to 55% of the MCLs. The magnetically labeled ARPE-19 cells (8 x 10(3) cells/mm(2), which corresponds to 10-fold the confluent concentration against the culture area [4 mm(2)]) were seeded into an ultra-low-attachment plate and a magnet (4000 G) was placed under the well. The magnetically labeled ARPE-19 cells formed an approximately 15-layered cell sheet after a 24 h of culture. When the magnet was removed, the sheets were detached from the bottom of the plate and then harvested and transferred to a tissue culture dish, using a magnet. Subsequently, the cell sheets were attached onto the dish, and the cells growing on the sheets were observed. This novel methodology, termed "magnetic force-based tissue engineering" (Mag-TE), is a possible approach for CNV treatment.
Authors:
Akira Ito; Eri Hibino; Chiaki Kobayashi; Hiroko Terasaki; Hideaki Kagami; Minoru Ueda; Takeshi Kobayashi; Hiroyuki Honda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tissue engineering     Volume:  11     ISSN:  1076-3279     ISO Abbreviation:  Tissue Eng.     Publication Date:    2005 Mar-Apr
Date Detail:
Created Date:  2005-05-04     Completed Date:  2005-08-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9505538     Medline TA:  Tissue Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  489-96     Citation Subset:  IM    
Affiliation:
Department of Biotechnology, School of Engineering, Nagoya University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Bioprosthesis
Cell Adhesion / physiology
Cell Culture Techniques / methods*
Cell Line
Cell Movement / physiology
Cell Polarity / physiology
Cells, Cultured
Feasibility Studies
Humans
Immunomagnetic Separation / methods*
Magnetics
Micromanipulation / methods*
Nanotubes
Pigment Epithelium of Eye / cytology*,  physiology*,  transplantation
Staining and Labeling / methods
Stress, Mechanical
Tissue Engineering / methods*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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