Document Detail


Construction and characterization of an infectious molecular clone of maedi-visna virus that expresses green fluorescent protein.
MedLine Citation:
PMID:  20447418     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The construction of a molecular clone of maedi-visna virus (MVV) expressing the enhanced green fluorescent protein (EGFP) is described. The egfp gene was inserted into the gene for dUTPase since it has been shown that dUTPase is dispensable for MVV replication both in vitro and in vivo. MVV-egfp is infectious and EGFP expression is stable over at least six passages. This fluorescent virus will be a useful tool for monitoring MVV infections.
Authors:
Hólmfrídur Sunna Gudmundsdóttir; Katrín Olafsdóttir; Sigrídur Rut Franzdóttir; Valgerdur Andrésdóttir
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-04
Journal Detail:
Title:  Journal of virological methods     Volume:  168     ISSN:  1879-0984     ISO Abbreviation:  J. Virol. Methods     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-07-26     Completed Date:  2010-11-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8005839     Medline TA:  J Virol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  98-102     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Affiliation:
Institute for Experimental Pathology, University of Iceland, Keldur v/Vesturlandsveg, Reykjavík, Iceland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Genomic Instability
Green Fluorescent Proteins / biosynthesis*,  genetics
Mutagenesis, Insertional
Pyrophosphatases / genetics
Recombination, Genetic
Serial Passage
Sheep
Staining and Labeling / methods*
Viral Proteins / genetics
Visna-maedi virus / genetics,  pathogenicity*
Chemical
Reg. No./Substance:
0/Viral Proteins; 0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins; EC 3.6.1.-/Pyrophosphatases; EC 3.6.1.23/dUTP pyrophosphatase

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