Document Detail

Construction and characterization of a NotI linking library of human chromosome 21.
MedLine Citation:
PMID:  1889809     Owner:  NLM     Status:  MEDLINE    
Effective procedures have been developed for constructing NotI linking libraries starting from chromosome-specific genomic libraries. Fifteen different single copy and two rDNA NotI linking clones from human chromosome 21 were identified in two libraries. Their chromosomal origin was confirmed, and regional location established using hybrid cell panels. Hybridization experiments with these probes revealed pairs of genomic NotI fragments, each ranging in size from less than 0.05 to 4.0 Mb. Many fragments displayed cell type variation. The total size of the NotI fragments detected in a human fibroblast cell line (GM6167) and mouse hybrid cell containing chromosome 21 as its only human component (WAV17) were approximately 32 and 34 Mb, respectively. If these fragments were all non-overlapping, this would correspond to about 70% of the 50-Mb content estimated for the whole chromosome. The linking clones will be enormously useful in the subsequent construction of a NotI restriction map of this chromosome. Characterization of these clones indicates the presence of numerous additional sites for other enzymes that recognize sequences containing CpG. Thus most NotI linking clones appear to derive from CpG islands and probably identify the 5' end of genes.
A Saito; J P Abad; D N Wang; M Ohki; C R Cantor; C L Smith
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genomics     Volume:  10     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  1991 Jul 
Date Detail:
Created Date:  1991-10-17     Completed Date:  1991-10-17     Revised Date:  2008-08-23    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  618-30     Citation Subset:  IM    
Department of Medicine (II), Niigata University School of Medicine, Japan.
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MeSH Terms
Cell Line
Chromosomes, Human, Pair 21*
Cloning, Molecular
Deoxyribonucleases, Type II Site-Specific*
Genetic Markers
Genetic Vectors
Genomic Library*
Hybrid Cells
Linkage (Genetics)
Restriction Mapping*
Grant Support
Reg. No./Substance:
0/Genetic Markers; EC, Type II Site-Specific; EC type II deoxyribonucleases

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