Document Detail

Construction of a cDNA library from microdissected guinea pig crista ampullaris.
MedLine Citation:
PMID:  8157507     Owner:  NLM     Status:  MEDLINE    
Poly(A) RNA was isolated from microdissected guinea pig crista ampullaris epithelium and converted into cDNA with RNase H- murine leukemia virus reverse transcriptase. After size fractionation, the cDNA was directionally ligated into the vector pSPORT 1 and the plasmids electroporated into E. coli. The library was found to have 1.6 x 10(7) independent colonies with 5% of the colonies lacking an insert. Thirty randomly selected colonies were checked for inserts and the average insert size was 833 base pairs with a range of 400 to 2300 base pairs. The library was screened with a beta-actin guinea pig cDNA probe and 0.16% of the colonies contained an insert hybridizing to the probe.
E R Wilcox; J Fex
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Hearing research     Volume:  73     ISSN:  0378-5955     ISO Abbreviation:  Hear. Res.     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-05-19     Completed Date:  1994-05-19     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7900445     Medline TA:  Hear Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  65-6     Citation Subset:  IM; S    
Laboratory of Molecular Biology, National Institute on Deafness and Other Communication Disorders, Bethesda, MD 20892.
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MeSH Terms
Actins / genetics
Base Composition
Cloning, Molecular
DNA Probes
DNA, Complementary / genetics*
Gene Library*
Guinea Pigs
Nucleic Acid Hybridization
Semicircular Canals / chemistry*
Reg. No./Substance:
0/Actins; 0/DNA Probes; 0/DNA, Complementary

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