| Construction, bacterial expression and characterization of a bifunctional single-chain antibody-phosphatase fusion protein targeted to the human erbB-2 receptor. | |
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MedLine Citation:
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PMID: 1369487 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have constructed genes expressing single-chain antigen binding proteins (scFv) which recognize the human erbB-2 receptor. These genes encode the heavy and light chain variable domains of an erbB-2 receptor specific monoclonal antibody, MAb FRP5, connected by a peptide linker. In order to express a bifunctional molecule, a bacterial alkaline phosphatase gene was fused 3' to the scFv gene. The scFv(FRP5) and scFv(FRP5)-alkaline phosphatase fusion protein (scFv(FRP5)-PhoA) expressed in E. coli specifically recognize the human erbB-2 protein and compete with MAb FRP5 for binding to the receptor. The bound scFv(FRP5)-PhoA protein can be detected directly on tumor cells using a substrate for alkaline phosphatase, showing that the chimeric protein retains both binding and enzymatic activity. |
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Authors:
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W Wels; I M Harwerth; M Zwickl; N Hardman; B Groner; N E Hynes |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Bio/technology (Nature Publishing Company) Volume: 10 ISSN: 0733-222X ISO Abbreviation: Biotechnology (N.Y.) Publication Date: 1992 Oct |
Date Detail:
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Created Date: 1992-11-06 Completed Date: 1992-11-06 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8309273 Medline TA: Biotechnology (N Y) Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1128-32 Citation Subset: B |
Affiliation:
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Friedrich Miescher Institute, Basel, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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genetics,
metabolism* Amino Acid Sequence Antibodies, Monoclonal / metabolism* Binding, Competitive Cloning, Molecular* Escherichia coli / genetics Female Genes, Bacterial Humans Immunohistochemistry Molecular Sequence Data Plasmids / chemistry*, genetics Proto-Oncogene Proteins / metabolism* Receptor, erbB-2 |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Proto-Oncogene Proteins; EC 2.7.10.1/Receptor, erbB-2; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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