| Construction of autologous human heart valves based on an acellular allograft matrix. | |
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MedLine Citation:
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PMID: 12354711 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Tissue engineered heart valves based on polymeric or xenogeneic matrices have several disadvantages, such as instability of biodegradable polymeric scaffolds, unknown transfer of animal related infectious diseases, and xenogeneic rejection patterns. To overcome these limitations we developed tissue engineered heart valves based on human matrices reseeded with autologous cells. METHODS AND RESULTS: Aortic (n=5) and pulmonary (n=6) human allografts were harvested from cadavers (6.2+/-3.1 hours after death) under sterile conditions. Homografts stored in Earle's Medium 199 enriched with 100 IU/mL Penicillin-Streptomycin for 2 to 28 days (mean 7.3+/-10.2 days) showed partially preserved cellular viability (MTT assay) and morphological integrity of the extracellular matrix (H-E staining). For decellularization, valves were treated with Trypsin/EDTA resulting in cell-free scaffolds (DNA-assay) with preserved extracellular matrix (confocal microscopy). Primary human venous endothelial cells (HEC) were cultivated and labeled with carboxy-fluorescein diacetate-succinimidyl ester in vitro. After recellularization under fluid conditions, EC were detected on the luminal surfaces of the matrix. They appeared as a monolayer of positively labeled cells for PECAM-1, VE-cadherin and Flk-1. Reseeded EC on the acellular allograft scaffold exhibited high metabolic activity (MTT assay). CONCLUSIONS: Earle's Medium 199 enriched with low concentration of antibiotics represents an excellent medium for long time preservation of extracellular matrix. After complete acellularization with Trypsin/EDTA, recellularization under shear stress conditions of the allogeneic scaffold results in the formation of a viable confluent HEC monolayer. These results represent a promising step toward the construction of autologous heart valves based on acellular human allograft matrix. |
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Authors:
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Serghei Cebotari; Heike Mertsching; Klaus Kallenbach; Sawa Kostin; Oleg Repin; Aurel Batrinac; Carmen Kleczka; Anatol Ciubotaru; Axel Haverich |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Circulation Volume: 106 ISSN: 1524-4539 ISO Abbreviation: Circulation Publication Date: 2002 Sep |
Date Detail:
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Created Date: 2002-09-30 Completed Date: 2002-10-21 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0147763 Medline TA: Circulation Country: United States |
Other Details:
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Languages: eng Pagination: I63-I68 Citation Subset: AIM; IM |
Affiliation:
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Leibniz Research Laboratory for Biotechnology and Artificial Organs, Hannover, Germany. serjciub@yahoo.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aortic Valve
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anatomy & histology,
chemistry,
metabolism Bioprosthesis* Cells, Cultured Collagen Type I / analysis Endothelium, Vascular / chemistry Extracellular Matrix / chemistry Heart Valve Prosthesis* Humans Microscopy, Fluorescence Pulmonary Valve / anatomy & histology, chemistry, metabolism Tissue Engineering / methods* Transplantation, Homologous |
| Chemical | |
Reg. No./Substance:
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0/Collagen Type I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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