Document Detail

Construction and analysis of cDNA library of Necator americanus third stage larvae.
MedLine Citation:
PMID:  12567467     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To obtain the genetic information on Necator americanus and to search for the purpose genes. METHODS: mRNA was isolated from the third stage larvae of Necator americanus maintained in hamsters. Double strand cDNA was synthesized and ligated to lambda ZAPII vector to construct the cDNA library. Expressed sequence tages (ESTs) were obtained by single pass sequencing of randomly isolated cDNA clones from the established library. RESULTS: A cDNA library of N. americanus was successfully constructed with high recombinant efficiency. The titer of unamplified library was 1 x 10(7). The insert size was about 750-3,000 bp. Of 11 ESTs obtained from the library, 7 have a significant homology with certain functional genes. CONCLUSION: A high quality and high representative cDNA library of N. americanus was constructed at the first time and some functional genes were identified from the library by ESTs.
B Zhan; J Hawdon; Q Shan; H Ren; H Qiang; S H Xiao; T H Li; Z Feng; P Hotez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases     Volume:  18     ISSN:  1000-7423     ISO Abbreviation:  Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi     Publication Date:  2000  
Date Detail:
Created Date:  2003-02-05     Completed Date:  2003-09-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8709992     Medline TA:  Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi     Country:  China    
Other Details:
Languages:  eng     Pagination:  26-8     Citation Subset:  IM    
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai, 200025.
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MeSH Terms
Cricetinae / parasitology
DNA, Complementary / genetics*
Expressed Sequence Tags
Gene Library*
Larva / genetics
Necator americanus / genetics*
RNA, Messenger / genetics,  isolation & purification
Grant Support
Reg. No./Substance:
0/DNA, Complementary; 0/RNA, Messenger

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