| Construction of Human Immunoglobulin Combinatorial Library and Screening of Phage Antibodies to Hepatitis B Surface Antigen. | |
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MedLine Citation:
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PMID: 12219225 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Human immunoglobulin combinatorial library was generated by using phage surface-display expression system, and phage antibodies (Fab fragments) to hepatitis B surface antigen (HbsAg) were screened from it. The products by half-nested PCR using signal peptide sequences as primers were superior in quality and quantity to those by PCR with conserved sequences in the 5'-end variable regions as primers. After three round of selections by biopanning, the ratio of positive clone was 69%. The inhibition assay showed the phage antibodies to be specifically anti-HbsAg. The V(H) genes were derived from V(H) I and V(H) III, while V(L)s belonged to V(lambda) II and V(lambda) I as shown by DNA sequencing. |
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Authors:
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Xue Wang; Hai-Tao Wang; Wan-Rong Chen; Jing Xu |
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Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Volume: 29 ISSN: 0582-9879 ISO Abbreviation: Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Publication Date: 1997 |
Date Detail:
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Created Date: 2002-Sep-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 20730160R Medline TA: Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) Country: - |
Other Details:
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Languages: ENG Pagination: 183-191 Citation Subset: - |
Affiliation:
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Institute of Mcriology Epidemology, Academy of Military Medical Sciences, Beijing 100071, China. |
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