Document Detail


Construction of high-density genetic linkage maps and mapping of growth-related quantitative trail loci in the Japanese flounder (Paralichthys olivaceus).
MedLine Citation:
PMID:  23209734     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
High-density genetic linkage maps were constructed for the Japanese flounder (Paralichthys olivaceus). A total of 1624 microsatellite markers were polymorphic in the reference family. Linkage analysis using JoinMap 4.0 resulted in the mapping of 1487 markers to 24 linkage groups, a result which was consistent with the 24 chromosomes seen in chromosome spreads. The female map was composed of 1257 markers, covering a total of 1663.8 cM with an average interval 1.35 cM between markers. The male map consisted of 1224 markers, spanning 1726.5 cM, with an average interval of 1.44 cM. The genome length in the Japanese flounder was estimated to be 1730.3 cM for the females and 1798.0 cM for the males, a coverage of 96.2% for the female and 96.0% for the male map. The mean recombination at common intervals throughout the genome revealed a slight difference between sexes, i.e. 1.07 times higher in the male than female. High-density genetic linkage maps are very useful for marker-assisted selection (MAS) programs for economically valuable traits in this species and for further evolutionary studies in flatfish and vertebrate species. Furthermore, four quantiative trait loci (QTL) associated with growth traits were mapped on the genetic map. One QTL was identified for body weight on LG 14 f, which explained 14.85% of the total variation of the body weight. Three QTL were identified for body width on LG14f and LG14m, accounting for 16.75%, 13.62% and 13.65% of the total variation in body width, respectively. The additive effects were evident as negative values. There were four QTL for growth traits clustered on LG14, which should prove to be very useful for improving growth traits using molecular MAS.
Authors:
Wentao Song; Renyi Pang; Yuze Niu; Fengtao Gao; Yongwei Zhao; Jing Zhang; Jian Sun; Changwei Shao; Xiaolin Liao; Lei Wang; Yongsheng Tian; Songlin Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-29
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-12-04     Completed Date:  2013-06-24     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e50404     Citation Subset:  IM    
Affiliation:
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Size
Chromosome Mapping / methods*
Female
Flounder / genetics*
Gene Library
Genetic Linkage*
Genetic Markers
Genome
Genotype
Lod Score
Male
Microsatellite Repeats / genetics
Models, Genetic
Phenotype
Polymorphism, Genetic
Quantitative Trait Loci
Sex Factors
Chemical
Reg. No./Substance:
0/Genetic Markers
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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