Document Detail

Construction and Expression of Food-Grade β-Galactosidase Gene in Lactococcus Lactis.
MedLine Citation:
PMID:  20924584     Owner:  NLM     Status:  In-Data-Review    
Recombinant Lactococcus lactis MG1363/pMG36e-lacZ exhibiting high β-galactosidase activities were constructed by us in the previous study. However, erythromycin resistance present in these recombinants restricted their practical application in food preparation. This study was conducted to delete the gene coding for erythromycin resistance present in recombinant L. lactis, as a result of which these bacteria express food-grade β-galactosidase. In this study, the recombinant plasmid pMG36e-lacZ was digested with restriction enzymes BclI and HpaI and the food-grade plasmid FGZW was rebuilt. FGZW was transformed into Escherichia coli JM109 and L. lactis MG1363. Erythromycin resistance, enzyme activity determination, gene sequencing and SDS-PAGE analysis indicated that these new recombinant bacteria lost erythromycin resistance and its relevant gene but still expressed β-galactosidase activities, although a decrease in the expression of β-galactosidase of these new strains was observed. The β-galactosidase food-grade expression system was successfully constructed and it could provide a new solution for the management of lactose intolerance. These results might promote the usage of gene-modified microorganisms and related technology in the food sector, which has the highest priority for food safety.
Wen Zhang; Chuan Wang; Chengyu Huang; Qian Yu; Hengchuan Liu; Chaowu Zhang; Xiaofang Pei
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Publication Detail:
Type:  Journal Article     Date:  2010-10-06
Journal Detail:
Title:  Current microbiology     Volume:  62     ISSN:  1432-0991     ISO Abbreviation:  Curr. Microbiol.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7808448     Medline TA:  Curr Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  639-44     Citation Subset:  IM    
Department of Nutrition and Food Hygiene, College of Public Health, Sichuan University, 16#, Section 3, Ren Min Nan Road, Chengdu, 610041, Sichuan, China.
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