| Construction and Application of a Food-Grade Expression System for Lactococcus lactis. | |
| | |
MedLine Citation:
|
PMID: 22674186 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
A food-grade host/vector expression system for Lactococcus lactis was constructed using alanine racemase gene (alr) as the complementation marker. We obtained an alanine racemase auxotrophic mutant L. lactis NZ9000Δalr by double-crossover recombination using temperature-sensitive integration plasmid pG(+)host9 and a food-grade vector pALR with entirely lactococcal DNA elements, including lactococcal replicon, nisin-inducible promoter PnisA and the alr gene from Lactobacillus casei BL23 as a complementation marker. By using the new food-grade host/vector system, the green fluorescent protein and capsid protein of porcine circovirus type II were successfully overexpressed under the nisin induction. These results indicate that this food-grade host/vector expression system has application potential as an excellent antigen delivery vehicle, and is also suitable for the use in the manufacture of ingredients for the food industry. |
| | |
Authors:
|
Wenwei Lu; Jian Kong; Wentao Kong |
Related Documents
:
|
12963396 - Eating tropical fruit reduces mercury exposure from fish consumption in the brazilian a... 15572226 - Mercury contamination in human hair and fish from cambodia: levels, specific accumulati... 18965716 - Determination of trace thorium using catalytic-adsorptive stripping voltammetry of the ... 1208186 - Mercury residues in breast muscle of wild ducks, 1970-71. 10751686 - Genomics, molecular genetics and the food industry. 20835936 - Intake of bisphenol a from canned beverages and foods on the belgian market. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-6-7 |
Journal Detail:
|
Title: Molecular biotechnology Volume: - ISSN: 1559-0305 ISO Abbreviation: - Publication Date: 2012 Jun |
Date Detail:
|
Created Date: 2012-6-7 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9423533 Medline TA: Mol Biotechnol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Efficiency of a new strategy involving a new class of natural hetero-ligand iron(III) chelates (Fe(I...
Next Document: Generation and Characterization of Yeast Two-Hybrid cDNA Libraries Derived From Two Distinct Mouse P...