Document Detail


Construction of a 1.2-Mb BAC/PAC contig of the porcine gene RYR1 region on SSC 6q1.2 and comparative analysis with HSA 19q13.13.
MedLine Citation:
PMID:  12376096     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We screened a porcine bacterial artificial chromosome (BAC) and a P1 derived artificial chromosome (PAC) library to construct a sequence-ready approximately 1.2-Mb BAC/PAC contig of the ryanodine receptor-1 gene (RYR1) region on porcine chromosome (SSC) 6q1.2. This genomic segment is of special interest because it harbors the locus for stress susceptibility in pigs and a putative quantitative trait locus for muscle growth. Detailed physical mapping of this gene-rich region allowed us to assign to this contig 17 porcine genes orthologous to known human chromosome 19 genes. Apart from the relatively well-characterized porcine gene RYR1, the other 16 genes represent novel chromosomal assignments and 14 genes have been cloned for the first time in pig. Comparative analysis of the porcine BAC/PAC contig with the human chromosome (HSA) 19q13.13 map revealed a completely conserved gene order of this segment between pig and human. A detailed porcine-human-mouse comparative map of this region was constructed.
Authors:
Flávia Martins-Wess; Rodja Voss-Nemitz; Cord Drögemüller; Bertram Brenig; Tosso Leeb
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genomics     Volume:  80     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-10-11     Completed Date:  2003-04-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  416-22     Citation Subset:  IM    
Affiliation:
Institute of Animal Breeding and Genetics, School of Veterinary Medicine Hannover, Bünteweg 17p, Hannover, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromosomes, Artificial, Bacterial*
Chromosomes, Artificial, P1 Bacteriophage*
Chromosomes, Human, Pair 19*
Humans
Physical Chromosome Mapping
Ryanodine Receptor Calcium Release Channel / genetics*
Swine
Synteny
Chemical
Reg. No./Substance:
0/Ryanodine Receptor Calcium Release Channel

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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