Document Detail

Constrictive pericarditis: detection of mycobacterium tuberculosis in paraffin-embedded pericardial tissues by polymerase chain reaction.
MedLine Citation:
PMID:  17303104     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Although the utility of polymerase chain reaction (PCR) for diagnosis of acute pleuro-pericardial tuberculosis has been well established, its use for chronic constrictive pericarditis is yet to be reported. AIMS: To define the sensitivity and specificity of PCR for diagnosis of tuberculosis (TB) in patients with constrictive pericarditis. METHODS: The medical records of 30 consecutive patients with constrictive pericarditis were reviewed. In addition their historical paraffin-embedded pericardial tissues were used for new histopathologic examination and PCR amplification for Mycobacterium tuberculosis genome. RESULTS: There were 23 males and 7 females with a mean age of 35+/-19.5 years. The anticipated causes of constriction included idiopathic (n=21), tuberculosis (n=5), cardiac surgery (n=2) and post traumatic (n=2). PCR became positive in nine patients. Four out of 5 patients with tuberculous granuloma had a positive test result. In addition all 4 patients with non-tuberculous constrictive pericarditis had a negative test result. Therefore considering the presence or absence of granuloma as a diagnostic criteria, the sensitivity and specificity of PCR were 4/5 (80%) and 20/25 (80%), respectively.
Mahmood Zamirian; Mehdi Mokhtarian; Mohammad Hosein Motazedian; Ahmad Monabati; Gholam Reza Rezaian
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Publication Detail:
Type:  Journal Article     Date:  2007-01-13
Journal Detail:
Title:  Clinical biochemistry     Volume:  40     ISSN:  0009-9120     ISO Abbreviation:  Clin. Biochem.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-02     Completed Date:  2007-06-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0133660     Medline TA:  Clin Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  355-8     Citation Subset:  IM    
Department of Internal Medicine (Cardiology), Namazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran.
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MeSH Terms
DNA, Bacterial / analysis,  genetics
Middle Aged
Mycobacterium tuberculosis / genetics*,  growth & development
Paraffin Embedding / methods
Pericarditis, Constrictive / complications*
Polymerase Chain Reaction / methods*
Reproducibility of Results
Sensitivity and Specificity
Tuberculosis / complications,  diagnosis*,  microbiology
Reg. No./Substance:
0/DNA, Bacterial

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