Document Detail

Constriction of rat extra-splenic veins to lipopolysaccharide involves endothelin-1.
MedLine Citation:
PMID:  20397012     Owner:  NLM     Status:  MEDLINE    
The spleen has an important role in blood volume regulation and increased resistance of post-capillary hilar veins (in mesentery adjoining the spleen) can regulate this. This study investigated whether venular constriction to lipopolysaccharide (LPS) involved endothelin-1 (ET-1). Pressure myography was used to study isolated extra-splenic (hilar) vessels from male Wistar rats (n = 111). Arteries and veins were treated with LPS (50 microg ml(-1)) for 4 h. Extra-splenic veins constricted to LPS (p < 0.05), but there was no effect on arteries. Denudation did not abolish venular constriction to LPS, indicating an endothelial independent mechanism. However, the dual ET-1 receptor antagonist bosentan (10(-5) M) and specific ET(A) and ET(B) antagonists ABT-627 (atrasentan, 6.3 x 10(-6) M) and A-192621(1.45 x 10(-6) M) completely abolished constriction of LPS-treated veins. ET-1 alone also constricted the extra-splenic arteries and veins (p < 0.05), with a greater response observed in veins (p < 0.05). ELISA also confirmed that serum and spleen levels of ET-1 increased in response to LPS (p < 0.05). That LPS-induced constriction of extra-splenic veins is mediated by ET-1. Greater constriction of post- versus pre-capillary extra-splenic vessels to LPS would result in increased intra-splenic fluid extravasation and hypovolaemia in vivo.
Arnaud Mansart; Lewis J Ruff; Mark P Ariaans; Jonathan J Ross; Charles S Reilly; Nicola J Brown; Susan Kaufman; Zoë L S Brookes
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-16
Journal Detail:
Title:  Naunyn-Schmiedeberg's archives of pharmacology     Volume:  381     ISSN:  1432-1912     ISO Abbreviation:  Naunyn Schmiedebergs Arch. Pharmacol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-13     Completed Date:  2010-08-16     Revised Date:  2013-06-18    
Medline Journal Info:
Nlm Unique ID:  0326264     Medline TA:  Naunyn Schmiedebergs Arch Pharmacol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  555-62     Citation Subset:  IM    
Academic Unit of Anaesthesia, University of Sheffield, Royal Hallamshire Hospital, Sheffield, S10 2RX, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Endothelin-1 / drug effects*,  metabolism
Enzyme-Linked Immunosorbent Assay
Hypovolemia / etiology
Lipopolysaccharides / toxicity*
Mesentery / metabolism
Pyrrolidines / pharmacology
Rats, Wistar
Receptor, Endothelin A / drug effects*,  metabolism
Spleen / drug effects,  metabolism
Sulfonamides / pharmacology
Vasoconstriction / drug effects*
Reg. No./Substance:
0/A 192621; 0/Endothelin-1; 0/Lipopolysaccharides; 0/Pyrrolidines; 0/Receptor, Endothelin A; 0/Sulfonamides; Q326023R30/bosentan; V6D7VK2215/atrasentan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Mechanical ventilation modulates Toll-like receptor signaling pathway in a sepsis-induced lung injur...
Next Document:  Transfer of Enteric Pathogens to Successive Habitats as Part of Microbial Cycles.