| Constriction of rat extra-splenic veins to lipopolysaccharide involves endothelin-1. | |
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MedLine Citation:
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PMID: 20397012 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The spleen has an important role in blood volume regulation and increased resistance of post-capillary hilar veins (in mesentery adjoining the spleen) can regulate this. This study investigated whether venular constriction to lipopolysaccharide (LPS) involved endothelin-1 (ET-1). Pressure myography was used to study isolated extra-splenic (hilar) vessels from male Wistar rats (n = 111). Arteries and veins were treated with LPS (50 microg ml(-1)) for 4 h. Extra-splenic veins constricted to LPS (p < 0.05), but there was no effect on arteries. Denudation did not abolish venular constriction to LPS, indicating an endothelial independent mechanism. However, the dual ET-1 receptor antagonist bosentan (10(-5) M) and specific ET(A) and ET(B) antagonists ABT-627 (atrasentan, 6.3 x 10(-6) M) and A-192621(1.45 x 10(-6) M) completely abolished constriction of LPS-treated veins. ET-1 alone also constricted the extra-splenic arteries and veins (p < 0.05), with a greater response observed in veins (p < 0.05). ELISA also confirmed that serum and spleen levels of ET-1 increased in response to LPS (p < 0.05). That LPS-induced constriction of extra-splenic veins is mediated by ET-1. Greater constriction of post- versus pre-capillary extra-splenic vessels to LPS would result in increased intra-splenic fluid extravasation and hypovolaemia in vivo. |
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Authors:
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Arnaud Mansart; Lewis J Ruff; Mark P Ariaans; Jonathan J Ross; Charles S Reilly; Nicola J Brown; Susan Kaufman; Zoë L S Brookes |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-16 |
Journal Detail:
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Title: Naunyn-Schmiedeberg's archives of pharmacology Volume: 381 ISSN: 1432-1912 ISO Abbreviation: Naunyn Schmiedebergs Arch. Pharmacol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-13 Completed Date: 2010-08-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0326264 Medline TA: Naunyn Schmiedebergs Arch Pharmacol Country: Germany |
Other Details:
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Languages: eng Pagination: 555-62 Citation Subset: IM |
Affiliation:
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Academic Unit of Anaesthesia, University of Sheffield, Royal Hallamshire Hospital, Sheffield, S10 2RX, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Endothelin-1 / drug effects*, metabolism Enzyme-Linked Immunosorbent Assay Hypovolemia / etiology Lipopolysaccharides / toxicity* Male Mesentery / metabolism Myography Pyrrolidines / pharmacology Rats Rats, Wistar Receptor, Endothelin A / drug effects*, metabolism Spleen / drug effects, metabolism Sulfonamides / pharmacology Vasoconstriction / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/A 192621; 0/Endothelin-1; 0/Lipopolysaccharides; 0/Pyrrolidines; 0/Receptor, Endothelin A; 0/Sulfonamides; 0/atrasentan; 147536-97-8/bosentan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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