Document Detail

Constitutive production of platelet-derived growth factor-like proteins by human prostate carcinoma cell lines.
MedLine Citation:
PMID:  2832055     Owner:  NLM     Status:  MEDLINE    
Prostate carcinoma cell lines DU-145 and PC-3 express both platelet derived growth factor (PDGF)-1 and PDGF-2/sis genes. Concomitantly, these cells synthesize and secrete PDGF-like proteins, as judged by indirect immunofluorescence and by direct immunoprecipitation with specific PDGF antiserum. Conditioned media derived from DU-145 and PC-3 cells stimulated the incorporation of [3H]thymidine by 3T3 cells and competed with 125I-labeled PDGF for its binding to cell surface receptors of 3T3 cells. The biological activity was stable to heating at 100 degrees C for 10 min, sensitive to reducing agents, and neutralized by the IgG fraction of PDGF antiserum, properties similar to those of authentic PDGF. Both DU-145 and PC-3 cell lines appear to lack receptors for PDGF as indicated by their inability to mitogenically respond to PDGF and receptor binding of 125I-labeled PDGF. Production of PDGF-like proteins by human prostate carcinoma cells may play an important role in a paracrine mode in the organization of the extracellular matrix of the malignant tissue.
N M Sitaras; E Sariban; M Bravo; P Pantazis; H N Antoniades
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  48     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1988 Apr 
Date Detail:
Created Date:  1988-04-22     Completed Date:  1988-04-22     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1930-5     Citation Subset:  IM    
Division of Biological Sciences, Harvard School of Public Health, Boston, Massachusetts.
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MeSH Terms
Carcinoma / metabolism*
Cell Line
Fluorescent Antibody Technique
Molecular Weight
Neoplasm Proteins / biosynthesis
Platelet-Derived Growth Factor / biosynthesis*
Prostatic Neoplasms / metabolism*
Proto-Oncogene Proteins / biosynthesis
RNA, Neoplasm / biosynthesis
Receptors, Cell Surface / metabolism
Receptors, Platelet-Derived Growth Factor
Transcription, Genetic
Grant Support
Reg. No./Substance:
0/Neoplasm Proteins; 0/Platelet-Derived Growth Factor; 0/Proto-Oncogene Proteins; 0/RNA, Neoplasm; 0/Receptors, Cell Surface; EC, Platelet-Derived Growth Factor

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