Document Detail


Constitutive mucin secretion linked to CFTR expression.
MedLine Citation:
PMID:  7524485     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mucus plugging is a hallmark of cystic fibrosis, but the link between the defective gene product, the cystic fibrosis transmembrane conductance regulator, and the abnormal mucus phenotype is unclear. To demonstrate CFTR involvement in mucin glycoprotein secretion in epithelial cells, a retroviral vector was used to overexpress CFTR in gallbladder epithelial cells, and constitutive mucin labeling and secretion were monitored. Achievement of high-level vector expression was confirmed by transduction with marker genes. Cells transduced with vectors carrying CFTR cDNA showed 5-fold increased expression of CFTR by Western blotting. Mucin labeling and secretion were 4-fold elevated in transduced cells. These results suggest constitutive mucin synthesis and secretion in gallbladder epithelial cells are regulated by CFTR.
Authors:
R Kuver; N Ramesh; S Lau; C Savard; S P Lee; W R Osborne
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  203     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1994 Sep 
Date Detail:
Created Date:  1994-10-31     Completed Date:  1994-10-31     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1457-62     Citation Subset:  IM    
Affiliation:
Department of Medicine, University of Washington, Seattle 98195.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antibodies
Blotting, Western
Cell Line
Chloride Channels / biosynthesis
Cystic Fibrosis Transmembrane Conductance Regulator
DNA, Complementary / metabolism
Dogs
Epithelium / metabolism
Gallbladder / metabolism*
Gene Expression*
Homeostasis
Humans
Kinetics
Membrane Proteins / analysis,  biosynthesis*
Molecular Sequence Data
Mucins / biosynthesis,  secretion*
Oligopeptides / chemistry,  immunology
Transfection
Grant Support
ID/Acronym/Agency:
DK 38531/DK/NIDDK NIH HHS; DK 41678/DK/NIDDK NIH HHS; DK 41978/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies; 0/CFTR protein, human; 0/Chloride Channels; 0/DNA, Complementary; 0/Membrane Proteins; 0/Mucins; 0/Oligopeptides; 126880-72-6/Cystic Fibrosis Transmembrane Conductance Regulator

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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