Document Detail

Constitutive and inducible nitric oxide synthases incorporate molecular oxygen into both nitric oxide and citrulline.
MedLine Citation:
PMID:  1721054     Owner:  NLM     Status:  MEDLINE    
Nitric oxide (NO) is synthesized by a number of cells from a guanidino nitrogen atom of L-arginine by the action of either constitutive or inducible NO synthases, both of which form citrulline as a co-product. We have determined the source of the oxygen in both NO and in citrulline formed by the constitutive NO synthase from the vascular endothelium and brain and by the inducible NO synthase from the murine macrophage cell line J774. All these enzymes incorporate molecular oxygen both into NO and into citrulline. Furthermore, activated J774 cells form NO from omega-hydroxyl-L-arginine, confirming the proposal that this compound is an intermediate in the biosynthesis of NO.
A M Leone; R M Palmer; R G Knowles; P L Francis; D S Ashton; S Moncada
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  266     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1991 Dec 
Date Detail:
Created Date:  1992-01-17     Completed Date:  1992-01-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  23790-5     Citation Subset:  IM    
Wellcome Research Laboratories, Beckenham, Kent, United Kingdom.
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MeSH Terms
Amino Acid Oxidoreductases / biosynthesis,  metabolism*
Brain / enzymology*
Cell Line
Citrulline / isolation & purification,  metabolism*
Cytosol / enzymology
Endothelium, Vascular / enzymology*
Enzyme Induction
Isotope Labeling / methods
Mass Spectrometry
Nitric Oxide / metabolism*
Nitric Oxide Synthase
Oxygen / metabolism*
Oxygen Isotopes
Reg. No./Substance:
0/Oxygen Isotopes; 10102-43-9/Nitric Oxide; 372-75-8/Citrulline; 7782-44-7/Oxygen; EC Oxide Synthase; EC 1.4.-/Amino Acid Oxidoreductases

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