| Constitutive and inducible nitric oxide synthases incorporate molecular oxygen into both nitric oxide and citrulline. | |
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MedLine Citation:
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PMID: 1721054 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nitric oxide (NO) is synthesized by a number of cells from a guanidino nitrogen atom of L-arginine by the action of either constitutive or inducible NO synthases, both of which form citrulline as a co-product. We have determined the source of the oxygen in both NO and in citrulline formed by the constitutive NO synthase from the vascular endothelium and brain and by the inducible NO synthase from the murine macrophage cell line J774. All these enzymes incorporate molecular oxygen both into NO and into citrulline. Furthermore, activated J774 cells form NO from omega-hydroxyl-L-arginine, confirming the proposal that this compound is an intermediate in the biosynthesis of NO. |
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Authors:
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A M Leone; R M Palmer; R G Knowles; P L Francis; D S Ashton; S Moncada |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 266 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1991 Dec |
Date Detail:
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Created Date: 1992-01-17 Completed Date: 1992-01-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 23790-5 Citation Subset: IM |
Affiliation:
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Wellcome Research Laboratories, Beckenham, Kent, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Oxidoreductases
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biosynthesis,
metabolism* Animals Aorta Brain / enzymology* Cell Line Citrulline / isolation & purification, metabolism* Cytosol / enzymology Endothelium, Vascular / enzymology* Enzyme Induction Isotope Labeling / methods Mass Spectrometry Mice Nitric Oxide / metabolism* Nitric Oxide Synthase Oxygen / metabolism* Oxygen Isotopes Rats Swine |
| Chemical | |
Reg. No./Substance:
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0/Oxygen Isotopes; 10102-43-9/Nitric Oxide; 372-75-8/Citrulline; 7782-44-7/Oxygen; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.4.-/Amino Acid Oxidoreductases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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