Document Detail


Constitutive expression of inducible nitric oxide synthase in human bronchial epithelial cells induces c-fos and stimulates the cGMP pathway.
MedLine Citation:
PMID:  7519434     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two major roles have been defined for nitric oxide (NO): cell-cell communication mediated by the stimulation of cyclic guanosine 3',5'-monophosphate (cGMP) synthesis and cytotoxicity by direct or indirect interaction of the free radical NO with cellular targets. Thus, pathologic states might result from an alteration of NO pathways, e.g., by deregulated activity of NO synthase. To investigate this hypothesis, we introduced the murine-inducible NO synthase (iNOS) sequence into immortalized human bronchial epithelial cells (BEAS-2B). iNOS activity, measured by conversion of [14C]arginine to [14C]citrulline in the presence of 1 mM EGTA, was higher than 100 pmol/min/mg protein in early passages of iNOS-transfected cells but decreased with cell subculturing. No iNOS activity could be detected in control vector-transfected cells. NO stimulated cGMP production in iNOS-transfected cells, and this effect was inhibited by the iNOS inhibitor NG-monomethyl-L-arginine. In addition, NO production induced c-fos expression and did not interfere with clonal cell growth. These results suggest that BEAS-2B cells constitute a suitable model to study the consequences of iNOS activity on signal transduction pathways in bronchial epithelium.
Authors:
E Felley-Bosco; S Ambs; C J Lowenstein; L K Keefer; C C Harris
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of respiratory cell and molecular biology     Volume:  11     ISSN:  1044-1549     ISO Abbreviation:  Am. J. Respir. Cell Mol. Biol.     Publication Date:  1994 Aug 
Date Detail:
Created Date:  1994-09-06     Completed Date:  1994-09-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8917225     Medline TA:  Am J Respir Cell Mol Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  159-64     Citation Subset:  IM    
Affiliation:
Laboratory of Human Carcinogenesis, National Cancer Institute, Bethesda, Maryland.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Oxidoreductases / biosynthesis*
Animals
Antigens, Polyomavirus Transforming / biosynthesis
Arginine / metabolism
Base Sequence
Blotting, Northern
Bronchi / metabolism*
Carbon Radioisotopes
Cell Division
Cell Line, Transformed
Citrulline / metabolism
Cyclic GMP / metabolism*
DNA Primers
Enzyme Induction
Epithelium / metabolism
Gene Expression*
Genes, fos*
Humans
Kinetics
Mice
Molecular Sequence Data
Nitric Oxide Synthase
Polymerase Chain Reaction
Proto-Oncogene Proteins c-fos / biosynthesis*
Simian virus 40 / genetics
Transfection
Chemical
Reg. No./Substance:
0/Antigens, Polyomavirus Transforming; 0/Carbon Radioisotopes; 0/DNA Primers; 0/Proto-Oncogene Proteins c-fos; 372-75-8/Citrulline; 74-79-3/Arginine; 7665-99-8/Cyclic GMP; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.4.-/Amino Acid Oxidoreductases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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