Document Detail


Constitutive expression of RyhB regulates the heme biosynthesis pathway and increases the 5-aminolevulinic acid accumulation in Escherichia coli.
MedLine Citation:
PMID:  24188714     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In the current study, the small RNA ryhB, which regulates the metabolism of iron in Escherichia coli, was constitutively expressed in engineered Escherichia coli DALA. The resulting strain Escherichia coli DALRA produced 16% more 5-aminolevulinic acid than the parent strain Escherichia coli DALA in batch fermentation. Meanwhile, we found that addition of iron in the medium increased heme formation and reduced 5-aminolevulinic acid yield, whereas the presence of iron chelator in the medium decreased heme concentration and increased the 5-aminolevulinic acid production efficiency (5-aminolevulinic acid yield /OD600 ). The qRT-PCR analysis showed that the mRNA levels of hemB and hemH were also decreased besides the known RyhB target genes of acnAB, sdhAB, fumA, and cydAB in Escherichia coli DALRA. These results indicated that small RNA can be used as a tool for regulating 5-aminolevulinic acid accumulation in Escherichia coli. This article is protected by copyright. All rights reserved.
Authors:
Fangfang Li; Yang Wang; Kai Gong; Qian Wang; Quanfeng Liang; Qingsheng Qi
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Publication Detail:
Type:  LETTER     Date:  2013-11-5
Journal Detail:
Title:  FEMS microbiology letters     Volume:  -     ISSN:  1574-6968     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2013 Nov 
Date Detail:
Created Date:  2013-11-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
This article is protected by copyright. All rights reserved.
Affiliation:
State Key Laboratory of Microbial Technology.
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