Document Detail


Constitutive IL-2 expression in HTLV-I-infected leukaemic T cell lines.
MedLine Citation:
PMID:  1710547     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An IL-2 autocrine growth circuit has been proposed as a major mechanism in HTLV-I-related leukaemogenesis. We have developed a polymerase chain reaction combined with reverse transcription RT-PCR to detect IL-2 transcripts and a sensitive immunostaining method for IL-2 protein. Combination of these two methods with in situ hybridization demonstrated that most cells of the T cell line IARC 301.5, whose proliferation is stimulated by autocrine IL-2, constitutively synthesize IL-2. This pattern was also found in the HTLV-I T cell lines HUT, MT2 and C 8166/45, and in the HTLV-I-negative T cell lines Jurkat and HSB2 but not MOLT4. Four non-lymphoid cell lines and cultured fibroblasts were negative, in agreement with the T cell specificity of IL-2 synthesis. Hence, most T cell lines tested, whether HTLV-I-infected or not, constitutively synthesize IL-2, suggesting a possible common feature of leukaemic T cell lines. The presence of IL-2 transcript and protein in most cells of the reacting cell lines is consistent with an autocrine process possibly involved at some stage in acquiring growth autonomy.
Authors:
J P Farcet; F Lebargy; C Lavignac; P Gaulard; A Dautry; L Gazzolo; P H Roméo; W Vainchenker
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  84     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  1991 Jun 
Date Detail:
Created Date:  1991-07-15     Completed Date:  1991-07-15     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  415-21     Citation Subset:  IM; X    
Affiliation:
INSERM U.91, Hôpital Henri Mondor, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / diagnostic use
Base Sequence
Humans
Interleukin-2 / analysis,  genetics*
Leukemia-Lymphoma, Adult T-Cell / immunology*
Molecular Sequence Data
Nucleic Acid Hybridization
Polymerase Chain Reaction
RNA, Messenger / analysis
RNA-Directed DNA Polymerase / genetics
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Interleukin-2; 0/RNA, Messenger; EC 2.7.7.49/RNA-Directed DNA Polymerase
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