| Constitutive IL-2 expression in HTLV-I-infected leukaemic T cell lines. | |
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MedLine Citation:
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PMID: 1710547 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An IL-2 autocrine growth circuit has been proposed as a major mechanism in HTLV-I-related leukaemogenesis. We have developed a polymerase chain reaction combined with reverse transcription RT-PCR to detect IL-2 transcripts and a sensitive immunostaining method for IL-2 protein. Combination of these two methods with in situ hybridization demonstrated that most cells of the T cell line IARC 301.5, whose proliferation is stimulated by autocrine IL-2, constitutively synthesize IL-2. This pattern was also found in the HTLV-I T cell lines HUT, MT2 and C 8166/45, and in the HTLV-I-negative T cell lines Jurkat and HSB2 but not MOLT4. Four non-lymphoid cell lines and cultured fibroblasts were negative, in agreement with the T cell specificity of IL-2 synthesis. Hence, most T cell lines tested, whether HTLV-I-infected or not, constitutively synthesize IL-2, suggesting a possible common feature of leukaemic T cell lines. The presence of IL-2 transcript and protein in most cells of the reacting cell lines is consistent with an autocrine process possibly involved at some stage in acquiring growth autonomy. |
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Authors:
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J P Farcet; F Lebargy; C Lavignac; P Gaulard; A Dautry; L Gazzolo; P H Roméo; W Vainchenker |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Clinical and experimental immunology Volume: 84 ISSN: 0009-9104 ISO Abbreviation: Clin. Exp. Immunol. Publication Date: 1991 Jun |
Date Detail:
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Created Date: 1991-07-15 Completed Date: 1991-07-15 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0057202 Medline TA: Clin Exp Immunol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 415-21 Citation Subset: IM; X |
Affiliation:
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INSERM U.91, Hôpital Henri Mondor, Paris, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Monoclonal
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diagnostic use Base Sequence Humans Interleukin-2 / analysis, genetics* Leukemia-Lymphoma, Adult T-Cell / immunology* Molecular Sequence Data Nucleic Acid Hybridization Polymerase Chain Reaction RNA, Messenger / analysis RNA-Directed DNA Polymerase / genetics Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Interleukin-2; 0/RNA, Messenger; EC 2.7.7.49/RNA-Directed DNA Polymerase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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