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THE CONSTITUTION OF MITOCHONDRIA AND MICROSOMES, AND THE DISTRIBUTION OF NUCLEIC ACID IN THE CYTOPLASM OF A LEUKEMIC CELL.
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MedLine Citation:
PMID:  19871395     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
1. Rat tumor extracts, containing chiefly the cytoplasmic constituents of leukemic cells, were fractionated into three main portions, the different components separating in the centrifuge according to size. 2. Mitochondria were isolated by centrifugation at relatively low speed. Elementary composition of purified mitochondria was found to correspond to about 11.5 per cent nitrogen, 1.6 per cent phosphorus, and 27 per cent lipids. Phosphorus and nitrogen content of the lipid portion suggests that as much as 75 to 80 per cent of the lipids of mitochondria is represented by phospholipids. Tests for ribose nucleic acid were positive. 3. Microsomes were separated by means of centrifugation at 18,000 x g. A relation between the high phosphorus content of the microsomes and the marked basophilia of the cytoplasm of leukemic cells is suggested. 4. Phosphorus distribution in the tumor extract, and light absorption analysis of the third fraction, seem to demonstrate that nucleic acid was not present either in a free condition, or in the form of nucleoprotein of relatively low molecular weight. The nature of the results suggests that ribose nucleic acid occurs in the cytoplasm of leukemic cells only in association with formed elements of relatively large size, namely microsomes, and mitochondria.
Authors:
A Claude
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of experimental medicine     Volume:  80     ISSN:  0022-1007     ISO Abbreviation:  J. Exp. Med.     Publication Date:  1944 Jul 
Date Detail:
Created Date:  2010-06-22     Completed Date:  2010-06-22     Revised Date:  2010-09-28    
Medline Journal Info:
Nlm Unique ID:  2985109R     Medline TA:  J Exp Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  19-29     Citation Subset:  -    
Affiliation:
Laboratories of The Rockefeller Institute for Medical Research.
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Full Text
Journal Information
Journal ID (nlm-ta): J Exp Med
ISSN: 0022-1007
ISSN: 1540-9538
Publisher: The Rockefeller University Press
Article Information
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Copyright © Copyright, 1944, by The Rockefeller Institute for Medical Research New York
Received Day: 13 Month: 3 Year: 1944
Print publication date: Day: 1 Month: 7 Year: 1944
Volume: 80 Issue: 1
First Page: 19 Last Page: 29
ID: 2135449
PubMed Id: 19871395

THE CONSTITUTION OF MITOCHONDRIA AND MICROSOMES, AND THE DISTRIBUTION OF NUCLEIC ACID IN THE CYTOPLASM OF A LEUKEMIC CELL
Albert Claude
From the Laboratories of The Rockefeller Institute for Medical Research


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