Document Detail

Constituents within pulp mill effluent deplete retinoid stores in white sucker and bind to rainbow trout retinoic acid receptors and retinoid X receptors.
MedLine Citation:
PMID:  14713038     Owner:  NLM     Status:  MEDLINE    
Wild female and male white sucker (Catostomus commersoni) inhabiting an area receiving pulp mill effluent had reduced hepatic levels of retinol, didehydroretinol, retinyl esters, and didehydroretinyl esters, while vitamin E levels were unaffected. This disruption of the retinoid system led us to test methanol and dichloromethane extracts from the effluent of 11 pulp mills from across Canada for their ability to bind to rainbow trout (Oncorhynchus mykiss) retinoic acid receptors (RARs) from the gill and retinoid X receptors (RXRs) from the liver. Concentrated extracts of the final effluent from 6 of the 11 pulp mills were able to displace greater than 25% of the receptor-bound [3H]all-trans retinoic acid (RA) or [3H]9-cis RA from trout RARs and RXRs, respectively. The ability of the extracts to displace retinoic acid did not appear to be linked to the pulping or treatment processes. Moreover, extracts with the greatest activity came from thermomechanical mills, suggesting the compounds may originate from the wood furnish. In addition, extracts prepared from wood furnish (wood chips: white spruce [50%], lodgepole pine [47%], and balsam fir [3%]) from one mill were able to displace [3H]RA from the RARs and RXRs. The 4-hydroxy RA, a metabolite of RA that has been shown to be generated in greater quantities in fish exposed to P450-inducing xenobiotics, was able to displace [3H]all-trans RA from trout RARs as effectively as unlabeled all-trans RA. These results suggest that pulp mill effluent may impact the retinoid system of fish at multiple sites, either by decreasing hepatic retinoid stores or through contributing additional ligands (from the wood furnish) that can bind to RA receptors.
Derek Alsop; Mark Hewitt; Mohan Kohli; Scott Brown; Glen Van Der Kraak
Related Documents :
9310738 - Oral isotretinoin. where now, where next?
10073058 - Acute promyelocytic leukemia. new methods in diagnosis and treatment.
12091498 - Hplc-ms/ms analysis of the products generated from all-trans-retinoic acid using recomb...
9885408 - Absorption and metabolism of (all-trans-) retinol acetate, retinol, and retinoic acid i...
15007068 - Molecular cloning and characterization of two mouse peroxisome proliferator-activated r...
33698 - Reactivity of the imino acids formed in the amino acid oxidase reaction.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Environmental toxicology and chemistry / SETAC     Volume:  22     ISSN:  0730-7268     ISO Abbreviation:  Environ. Toxicol. Chem.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2004-01-09     Completed Date:  2004-04-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8308958     Medline TA:  Environ Toxicol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2969-76     Citation Subset:  IM    
Department of Zoology, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Animals, Wild
Cypriniformes / physiology*
Environmental Exposure*
Industrial Waste*
Liver / physiology
Oncorhynchus mykiss / physiology*
Receptors, Retinoic Acid / drug effects,  physiology*
Retinoid X Receptors
Transcription Factors / drug effects,  physiology*
Reg. No./Substance:
0/Industrial Waste; 0/Ligands; 0/Receptors, Retinoic Acid; 0/Retinoid X Receptors; 0/Transcription Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A comparative assessment of azinphosmethyl bioaccumulation and toxicity in two estuarine meiobenthic...
Next Document:  Biotransformation and genotoxicity of fluoranthene in the deposit-feeding polychaete Capitella sp. I...