| Constituents from Cistus salvifolius (Cistaceae) Activate Peroxisome Proliferator-Activated Receptor-γ but Not -δ and Stimulate Glucose Uptake by Adipocytes. | |
| | |
MedLine Citation:
|
PMID: 20922652 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
A number of medicinal/culinary herbs have been reported to improve glucose metabolism and to yield hypoglycemic effects in patients with diabetes. Since stimulation of insulin sensitivity appears to be a potential mechanism, peroxisome proliferator-activated receptor (PPAR) γ is a likely target molecule for small lipophilic compounds derived from endogenous metabolism and nutrition. Functionally, PPAR γ integrates the control of energy, lipid, and glucose homeostasis. In addition, PPAR δ activity is involved in energy expenditure. Therefore the aim of this study was to investigate whether PPAR γ and PPAR δ as well as the stimulation of glucose uptake is activated by botanical products. CISTUS SALVIFOLIUS (Cistaceae) has been identified as a candidate botanical in a preliminary screening of extracts from medicinal plants of Greek flora. In a bioguided approach, crude extracts, fractions and in the end purified compounds have been evaluated for PPAR γ and PPAR δ specific activities using cell-based transactivation assays. Glucose uptake was measured by nonradioactive 2-[ N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxyglucose (2-NBDG) uptake. Concerning PPAR γ several extracts induced reporter gene activity, and clear dose-response patterns (0.1-100 µg/mL) could be established in the case of the cyclohexane and dichloromethane extracts. Isolation of individual compounds from the cyclohexane extract revealed that at least 6 out of 7 compounds isolated were active with TRANS-cinnamic acid showing a clear dose-response pattern. In contrast, they were found to be inactive on PPAR δ. The same compounds, however, were also active in stimulating glucose uptake into 3T3-L1 adipocytes. In summary, the bioguided fractionation of CISTUS SALVIFOLIUS yields PPAR γ stimulating metabolites with differing chemical natures. In conclusion, PPAR γ represents a candidate molecule for the mediation of improvement of glucose metabolism by botanical/nutritional products. |
| | |
Authors:
|
Claudia Kühn; Niki Eliza Arapogianni; Maria Halabalaki; Jana Hempel; Nicole Hunger; Jannette Wober; Alexios Leandros Skaltsounis; Günter Vollmer |
Publication Detail:
|
Type: Journal Article Date: 2010-10-04 |
Journal Detail:
|
Title: Planta medica Volume: 77 ISSN: 1439-0221 ISO Abbreviation: Planta Med. Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-03-08 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0066751 Medline TA: Planta Med Country: Germany |
Other Details:
|
Languages: eng Pagination: 346-53 Citation Subset: IM |
Copyright Information:
|
© Georg Thieme Verlag KG Stuttgart · New York. |
Affiliation:
|
Molecular Cell Physiology & Endocrinology, Technische Universität Dresden, Dresden, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Protective, Antioxidative and Antiapoptotic Effects of 2-Methoxy-6-Acetyl-7-Methyljuglone from Polyg...
Next Document: Cytotoxic Aporphine Alkaloids from Ocotea acutifolia.