| Consomic strategies to localize genomic regions related to vascular reactivity in the Dahl salt-sensitive rat. | |
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MedLine Citation:
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PMID: 16772359 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chromosomal substitution strains afford the opportunity to discover regions of the rat genome that contain genes related to cardiovascular traits with the long-range goal of linking these genes to physiological function. PhysGen (Programs for Genomic Applications) created a consomic panel of rats derived from the introgression of a single chromosome (> or =95% of the BN chromosome, one at a time) of the Brown Norway (BN/NHsdMcwi) rat onto the homogeneous genetic background of the Dahl salt-sensitive rat (SS/JrHsdMcwi). For 3 wk before the experiment, the rats were maintained on a low-salt diet (0.4% NaCl). The dose response of aortic rings from each strain of rat to phenylephrine, acetylcholine, sodium nitroprusside, and three different levels of tissue bath hypoxia (10, 5, and 0% O2) was measured and compared with the parental SS rat. To maximize the possibility that differences among the strains would become apparent, each strain of rat including the parental SS and BN was also studied after being maintained on a high-salt diet (4.0% NaCl) for 3 wk. If the response of the aortic ring from a consomic strain to these vasoactive substances was different from that of the SS parental strain, it was concluded that the introgressed chromosome contained a gene or genes that contributed to that difference. Because the BN chromosome is removed from its native background and the SS rat loses a native chromosome, it is also necessary to consider the contribution of changes in gene-to-gene interaction. |
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Authors:
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Mary Pat Kunert; Ines Drenjancevic-Peric; Melinda R Dwinell; Julian H Lombard; Allen W Cowley; Andrew S Greene; Anne E Kwitek; Howard J Jacob |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, N.I.H., Extramural Date: 2006-06-13 |
Journal Detail:
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Title: Physiological genomics Volume: 26 ISSN: 1531-2267 ISO Abbreviation: Physiol. Genomics Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2006-08-17 Completed Date: 2006-10-10 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9815683 Medline TA: Physiol Genomics Country: United States |
Other Details:
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Languages: eng Pagination: 218-25 Citation Subset: IM |
Affiliation:
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University of Wisconsin-Milwaukee, College of Nursing, Milwaukee, Wisconsin 53211, USA. mpkunert@uwm.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcholine
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pharmacology Animals Aorta / drug effects, metabolism, physiology Blood Pressure / drug effects, genetics, physiology Blood Vessels / drug effects, metabolism, physiology* Chromosome Mapping / methods* Chromosomes, Mammalian / genetics Diet, Sodium-Restricted Dose-Response Relationship, Drug Female Male Nitroprusside / pharmacology Oxygen / pharmacology Rats Rats, Inbred BN Rats, Inbred Dahl Sodium Chloride, Dietary / administration & dosage Vasoconstriction / drug effects |
| Grant Support | |
ID/Acronym/Agency:
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U01 HL-66579/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Sodium Chloride, Dietary; 15078-28-1/Nitroprusside; 51-84-3/Acetylcholine; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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