Document Detail

Conserved and variable features of Gag structure and arrangement in immature retrovirus particles.
MedLine Citation:
PMID:  20810738     Owner:  NLM     Status:  MEDLINE    
The assembly of retroviruses is driven by oligomerization of the Gag polyprotein. We have used cryo-electron tomography together with subtomogram averaging to describe the three-dimensional structure of in vitro-assembled Gag particles from human immunodeficiency virus, Mason-Pfizer monkey virus, and Rous sarcoma virus. These represent three different retroviral genera: the lentiviruses, betaretroviruses and alpharetroviruses. Comparison of the three structures reveals the features of the supramolecular organization of Gag that are conserved between genera and therefore reflect general principles of Gag-Gag interactions and the features that are specific to certain genera. All three Gag proteins assemble to form approximately spherical hexameric lattices with irregular defects. In all three genera, the N-terminal domain of CA is arranged in hexameric rings around large holes. Where the rings meet, 2-fold densities, assigned to the C-terminal domain of CA, extend between adjacent rings, and link together at the 6-fold symmetry axis with a density, which extends toward the center of the particle into the nucleic acid layer. Although this general arrangement is conserved, differences can be seen throughout the CA and spacer peptide regions. These differences can be related to sequence differences among the genera. We conclude that the arrangement of the structural domains of CA is well conserved across genera, whereas the relationship between CA, the spacer peptide region, and the nucleic acid is more specific to each genus.
Alex de Marco; Norman E Davey; Pavel Ulbrich; Judith M Phillips; Vanda Lux; James D Riches; Tibor Fuzik; Tomas Ruml; Hans-Georg Kräusslich; Volker M Vogt; John A G Briggs
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-09-01
Journal Detail:
Title:  Journal of virology     Volume:  84     ISSN:  1098-5514     ISO Abbreviation:  J. Virol.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-26     Completed Date:  2010-11-22     Revised Date:  2011-07-28    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  11729-36     Citation Subset:  IM    
European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
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MeSH Terms
Amino Acid Sequence
Cell Line
Conserved Sequence
Gene Products, gag / chemistry*,  genetics,  metabolism
HIV-1 / chemistry*,  genetics,  physiology
Mason-Pfizer monkey virus / chemistry*,  genetics,  physiology
Molecular Sequence Data
Protein Structure, Tertiary
Rous sarcoma virus / chemistry*,  genetics,  physiology
Sequence Alignment
Virion / chemistry,  genetics,  physiology*
Virus Assembly
Grant Support
Reg. No./Substance:
0/Gene Products, gag

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