Document Detail


Conserved P-TEFb-interacting domain of BRD4 inhibits HIV transcription.
MedLine Citation:
PMID:  17690245     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have identified a conserved region in the C-terminal domain of bromodomain-containing protein 4 (BRD4) that mediates its specific interaction with positive transcription elongation factor b (P-TEFb). This domain is highly conserved in testis-specific bromodomain protein (BRDT) and Drosophila fs(1)h. Both BRDT and fs(1)h specifically interact with P-TEFb in mammalian cells, and this interaction depends on their C-terminal domains. Overexpression of the BRD4 P-TEFb-interacting domain disrupts the interaction between the HIV transactivator Tat and P-TEFb and suppresses the ability of Tat to transactivate the HIV promoter. Incubation of cells with a synthetic peptide containing the C-terminal domain of BRD4 interferes with transactivation of the HIV promoter by the Tat protein.
Authors:
Dwayne A Bisgrove; Tokameh Mahmoudi; Peter Henklein; Eric Verdin
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-08-09
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  104     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-08-23     Completed Date:  2007-10-19     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  13690-5     Citation Subset:  IM    
Affiliation:
Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA 94158, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Cell Line
Conserved Sequence
Gene Products, tat / genetics,  metabolism
HIV / genetics*
Humans
Molecular Sequence Data
Nuclear Proteins / chemistry,  genetics,  metabolism*
Positive Transcriptional Elongation Factor B / genetics,  metabolism*
Promoter Regions, Genetic / genetics
Protein Binding
Protein Subunits / genetics,  metabolism
Sequence Alignment
Transcription Factors / chemistry,  genetics,  metabolism*
Transcription, Genetic / genetics*
Transcriptional Activation / genetics
tat Gene Products, Human Immunodeficiency Virus
Grant Support
ID/Acronym/Agency:
P30 AI27763/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/BRD4 protein, human; 0/Gene Products, tat; 0/Nuclear Proteins; 0/Protein Subunits; 0/Transcription Factors; 0/tat Gene Products, Human Immunodeficiency Virus; EC 2.7.11.-/Positive Transcriptional Elongation Factor B
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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