| Conserved eukaryotic fusogens can fuse viral envelopes to cells. | |
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MedLine Citation:
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PMID: 21436398 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Caenorhabditis elegans proteins AFF-1 and EFF-1 [C. elegans fusion family (CeFF) proteins] are essential for developmental cell-to-cell fusion and can merge insect cells. To study the structure and function of AFF-1, we constructed vesicular stomatitis virus (VSV) displaying AFF-1 on the viral envelope, substituting the native fusogen VSV glycoprotein. Electron microscopy and tomography revealed that AFF-1 formed distinct supercomplexes resembling pentameric and hexameric "flowers" on pseudoviruses. Viruses carrying AFF-1 infected mammalian cells only when CeFFs were on the target cell surface. Furthermore, we identified fusion family (FF) proteins within and beyond nematodes, and divergent members from the human parasitic nematode Trichinella spiralis and the chordate Branchiostoma floridae could also fuse mammalian cells. Thus, FF proteins are part of an ancient family of cellular fusogens that can promote fusion when expressed on a viral particle. |
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Authors:
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Ori Avinoam; Karen Fridman; Clari Valansi; Inbal Abutbul; Tzviya Zeev-Ben-Mordehai; Ulrike E Maurer; Amir Sapir; Dganit Danino; Kay Grünewald; Judith M White; Benjamin Podbilewicz |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-03-24 |
Journal Detail:
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Title: Science (New York, N.Y.) Volume: 332 ISSN: 1095-9203 ISO Abbreviation: Science Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-04-29 Completed Date: 2011-05-12 Revised Date: 2012-06-11 |
Medline Journal Info:
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Nlm Unique ID: 0404511 Medline TA: Science Country: United States |
Other Details:
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Languages: eng Pagination: 589-92 Citation Subset: IM |
Affiliation:
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Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Arthropods / chemistry Biological Evolution Caenorhabditis elegans / chemistry Caenorhabditis elegans Proteins / chemistry, genetics, metabolism*, ultrastructure Cell Fusion* Cell Line Cell Membrane / metabolism* Chordata, Nonvertebrate / chemistry Ctenophora / chemistry Membrane Fusion* Membrane Glycoproteins / chemistry, genetics, metabolism* Molecular Sequence Data Naegleria fowleri / chemistry Nematoda / chemistry Recombinant Proteins / metabolism Recombination, Genetic Vesicular stomatitis Indiana virus / genetics, physiology*, ultrastructure Viral Envelope Proteins / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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090895//Wellcome Trust; AI22470/AI/NIAID NIH HHS; R01 AI022470-24/AI/NIAID NIH HHS; //Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/AFF-1 protein, C elegans; 0/Caenorhabditis elegans Proteins; 0/EFF-1 protein, C elegans; 0/Membrane Glycoproteins; 0/Recombinant Proteins; 0/Viral Envelope Proteins |
| Comments/Corrections | |
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