Document Detail

Conserved eukaryotic fusogens can fuse viral envelopes to cells.
MedLine Citation:
PMID:  21436398     Owner:  NLM     Status:  MEDLINE    
Caenorhabditis elegans proteins AFF-1 and EFF-1 [C. elegans fusion family (CeFF) proteins] are essential for developmental cell-to-cell fusion and can merge insect cells. To study the structure and function of AFF-1, we constructed vesicular stomatitis virus (VSV) displaying AFF-1 on the viral envelope, substituting the native fusogen VSV glycoprotein. Electron microscopy and tomography revealed that AFF-1 formed distinct supercomplexes resembling pentameric and hexameric "flowers" on pseudoviruses. Viruses carrying AFF-1 infected mammalian cells only when CeFFs were on the target cell surface. Furthermore, we identified fusion family (FF) proteins within and beyond nematodes, and divergent members from the human parasitic nematode Trichinella spiralis and the chordate Branchiostoma floridae could also fuse mammalian cells. Thus, FF proteins are part of an ancient family of cellular fusogens that can promote fusion when expressed on a viral particle.
Ori Avinoam; Karen Fridman; Clari Valansi; Inbal Abutbul; Tzviya Zeev-Ben-Mordehai; Ulrike E Maurer; Amir Sapir; Dganit Danino; Kay Grünewald; Judith M White; Benjamin Podbilewicz
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-03-24
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  332     ISSN:  1095-9203     ISO Abbreviation:  Science     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-04-29     Completed Date:  2011-05-12     Revised Date:  2014-03-27    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  589-92     Citation Subset:  IM    
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MeSH Terms
Amino Acid Sequence
Arthropods / chemistry
Biological Evolution
Caenorhabditis elegans / chemistry
Caenorhabditis elegans Proteins / chemistry,  genetics,  metabolism*,  ultrastructure
Cell Fusion*
Cell Line
Cell Membrane / metabolism*
Chordata, Nonvertebrate / chemistry
Ctenophora / chemistry
Membrane Fusion*
Membrane Glycoproteins / chemistry,  genetics,  metabolism*
Molecular Sequence Data
Naegleria fowleri / chemistry
Nematoda / chemistry
Recombinant Proteins / metabolism
Recombination, Genetic
Vesicular stomatitis Indiana virus / genetics,  physiology*,  ultrastructure
Viral Envelope Proteins / metabolism
Grant Support
090532//Wellcome Trust; 090895//Wellcome Trust; AI22470/AI/NIAID NIH HHS; R01 AI022470/AI/NIAID NIH HHS; R01 AI022470-24/AI/NIAID NIH HHS; //Wellcome Trust
Reg. No./Substance:
0/AFF-1 protein, C elegans; 0/Caenorhabditis elegans Proteins; 0/EFF-1 protein, C elegans; 0/Membrane Glycoproteins; 0/Recombinant Proteins; 0/Viral Envelope Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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